Advertisement

If you have an ACS member number, please enter it here so we can link this account to your membership. (optional)

ACS values your privacy. By submitting your information, you are gaining access to C&EN and subscribing to our weekly newsletter. We use the information you provide to make your reading experience better, and we will never sell your data to third party members.

ENJOY UNLIMITED ACCES TO C&EN

Analytical Chemistry

More Coverage For Glucose Meters

Techniques replace functional DNAs with antibodies to analyze a wider range of targets

April 23, 2012 | A version of this story appeared in Volume 90, Issue 17

[+]Enlarge
Credit: Courtesy of Li Huey, Yu Xiang, and Yi Lu
Xiang and Lu’s antibody sandwich assay is one of two approaches they developed to allow commercial meters to quantitate proteins and other analytes.
A schematic showing how the sandwich assay detects analytes by binding to them and converting sucrose to glucose.
Credit: Courtesy of Li Huey, Yu Xiang, and Yi Lu
Xiang and Lu’s antibody sandwich assay is one of two approaches they developed to allow commercial meters to quantitate proteins and other analytes.

An antibody-based strategy has considerably widened the range of analytes that can be detected with personal glucose meters, according to new work by Yu Xiang and Yi Lu at the University of Illinois, Urbana-Champaign (Anal. Chem., DOI: 10.1021/ac300517n). Last year, the same team used DNAzymes and aptamers, functional DNAs that bind specific compounds, to enable commercially available personal glucose meters to measure a range of targets (C&EN, July 25, 2011, page 9). But functional DNAs have been developed for only a limited number of target compounds. Now, Xiang and Lu have replaced functional DNAs with antibodies that have been developed to recognize a much wider range of targets. They use sandwich and competitive antibody assays to quantitate a diagnostic protein (prostate-specific antigen) and a toxin (ochratoxin A), respectively. In both approaches, the antibody recognizes the analyte, and antibody-associated invertase converts sucrose to glucose, which is measured by the glucose meter. With support from the National Science Foundation’s Innovation Corps, Lu has founded a company, GlucoSentient, that will commercialize glucose-meter-based tests for nonglucose analytes.

Article:

This article has been sent to the following recipient:

0 /1 FREE ARTICLES LEFT THIS MONTH Remaining
Chemistry matters. Join us to get the news you need.