Advertisement

If you have an ACS member number, please enter it here so we can link this account to your membership. (optional)

ACS values your privacy. By submitting your information, you are gaining access to C&EN and subscribing to our weekly newsletter. We use the information you provide to make your reading experience better, and we will never sell your data to third party members.

ENJOY UNLIMITED ACCES TO C&EN

Environment

What You Missed When Chemists Gathered In Hawaii

Pacifichem News: A taste of the happenings and the research presented during last month’s meeting in Hawaii

by Stephen K. Ritter , Matt Davenport , Bethany Halford , Stu Borman
January 4, 2016 | A version of this story appeared in Volume 94, Issue 1

VIEW FROM THE TOP
This is a view from near the ceiling of scientists milling around a poster session at Pacifichem.
Credit: Matt Davenport/C&EN
Looking down on a poster session at Pacifichem 2015.

It’s been said among gossip circles that the International Chemical Congress of Pacific Basin Societies—or Pacifichem, as it’s more commonly called—is vacation time for chemists. Every five years, chemists gather in Honolulu to network, to present cutting-edge research—and maybe to catch a few waves.

RED ALERT
[+]Enlarge
Credit: Mitch Jacoby/C&EN
One of the many signs throughout Honolulu warning that “the chemists” have arrived.
This is a photo of a Pacifichem banner hanging on the street in Honolulu with palm trees in the background.
Credit: Mitch Jacoby/C&EN
One of the many signs throughout Honolulu warning that “the chemists” have arrived.

During this year’s congress, held Dec. 15–20, even Peter J. Stang, the 2015 Pacifichem chair, acknowledged the rumor. Stang, who is a professor at the University of Utah and editor-in-chief of the Journal of the American Chemical Society, reminded attendees at the opening ceremony that sharks cruise the waters surrounding Hawaii. They’re particularly fond of chemists, he quipped, so attending poster sessions and symposia instead of hanging ten “could just save your lives.”

Despite the draw of sunshine and surf, chemists gave approximately 17,000 oral presentations during 330 sessions this year. A good number fit with the meeting’s theme, “Chemical Networking: Building Bridges Across The Pacific,” which emphasized the collaborative nature of chemistry and encouraged networking among members of the seven chemical organizations that run Pacifichem: the American Chemical Society, the Canadian Society for Chemistry, the Chemical Society of Japan, the Chinese Chemical Society, the Korean Chemical Society, the New Zealand Institute of Chemistry, and the Royal Australian Chemical Institute. Seventy-four companies and organizations participated as exhibitors at the conference expo.

But of course it wasn’t all business at Pacifichem: Attendees enjoyed Polynesian dance performances during and after the opening ceremony, and they took in concerts by Chemistry Orchestra throughout the week. Founded in 2003 in Tokyo and made up of volunteer chemists, the ensemble played classical music at a Pacifichem poster session, a luncheon, and the closing ceremony.

REUNITED
This is a photo of scientists standing in front of a Christmas tree.
Credit: Matt Davenport/C&EN
Current and former members of Aiko Fukazawa’s group at Nagoya University catch up at Pacifichem. From left to right: Naoya Suzuki, Hiroshi Osaki, Hiroaki Ogasawara, Soren Mellerup (now at Queen’s University in Ontario), Saki Azeyanagi, Fukazawa, and Cuihua Zhao (now at Shandong University).

In between taking in the sunshine, sights, and sounds, C&EN took in the science Pacifichem had to offer. Here, we give you a taste of some of the notable research presented.


THE NEW SWITCHEROO
A scheme shows how the switchable antibiotic works.
Credit: Angew. Chem. Int. Ed.
The antimicrobial activity of a polymer (PPV) can be switched off with cucurbit[7]uril (CB[7]) and back on again with amantadine.

Switch-hitting Against Bacteria

As infectious bacteria continue to evolve defenses against conventional antibiotics, many people are focused on the need for more potent drugs. But Shu Wang of the Chinese Academy of Sciences presented a different route for fighting antibiotic resistance during this year’s Pacifichem meeting. Wang and his team have developed polymers with antimicrobial activity that can essentially be turned on and off (Angew. Chem. Int. Ed. 2015, DOI: 10.1002/anie.201504566).

The polymers are actively antimicrobial only when they need to be, so they don’t continually pressure bacteria to evolve resistance, Wang said. Making switchable antibiotics isn’t a new strategy: Researchers have already investigated compounds that can be switched on or off using light. Wang’s antibiotics use reversible supramolecular chemistry that also works in the dark, he told C&EN. These biocides are cationic polymers derived from poly(phenylene vinylene) that have linear backbones with cationic ammonium arms for controlling the material’s antibiotic properties. The positively charged arms help kill bacteria by penetrating cell membranes. To switch off the antimicrobial activity, Wang’s team exposes the polymers to ring-shaped cucurbit[7]uril molecules, or CB[7]. These compounds cuff the ammonium arms, hindering the polymer’s ability to latch on to bacteria. To restore the polymer’s antimicrobial activity, the team adds the small molecule amantadine to the bacterial system, usually a culture of Escherichia coli. Amantadine bonds with CB[7], removing the cuffs from the polymers. When it comes to combating antibiotic resistance, “innovative approaches should be applauded,” said Ben L. Feringa of the University of Groningen, who was not involved in this study but pioneered earlier examples of switchable antibiotics. Feringa, though, has a hard time imagining how this multiple-component process would be administered to patients in the clinic. Wang is optimistic, however, and he pointed out that the polymers could also be used to deactivate antimicrobials before they are released into the environment, for instance, in wastewater from agriculture.Matt Davenport

THREE THE HARD WAY
A set of vials show the segregation of molecules trapped in cage molecules into three different phases.
Credit: Angew. Chem. Int. Ed.
Three different cage molecules that are preferentially soluble in different immiscible solvents selectively trap a different molecule when a three-component mixture is added.

Self-assembling Molecular Prisons

OPEN-AND-SHUT CASE
Reaction scheme shows how a metal-organic cage is reversibly opened and closed to trap a fullerene molecule.
Credit: ACS Cent. Sci.
A cage molecule traps a fullerene and uses a catalytic phosphine “fuel” to keep the assembly open and the fullerene free; when the fuel runs out, the assembly closes with the fullerene trapped back inside.

Functional nanomaterials based on metal-organic coordination chemistry are the forte of Jonathan R. Nitschke and his group at the University of Cambridge. These researchers have been developing self-assembling cage molecules that are designed to reversibly trap pollutants, store gases, separate mixtures, and controllably release drugs. One of the Nitschke group’s latest efforts, presented at Pacifichem, is a triphasic sorting system in which three different molecular cages partition into three different immiscible solvent layers in the same flask. When a three-compound mixture is added, the cages each selectively trap a different guest compound and segregate them into the different layers (Angew. Chem. Int. Ed. 2015, DOI: 10.1002/anie.201505774).

The team pulled this trifecta off by building a pyramid-shaped iron cage, using diamine and formylpyridine organic components, that is soluble in water and selectively binds 1-fluorobenzene; a cube-shaped iron cage, using a bis(pyridylimine) organic component, that is soluble in a phosphonium-based ionic liquid and selectively binds 9-trifluoroacetylanthracene; and another pyramid-shaped iron cage, using triamine and pyridinecarboxaldehyde components, that is soluble in an imidazole-based ionic liquid and selectively binds 1-fluoroadamantane. Adding an acid unlocks the cages and releases the trapped molecules.

On another front, Nitschke’s group has prepared metallosupramolecular cage systems that can be opened and closed to release and trap guest molecules for controlled periods of time by catalytic binding of a ligand (ACS Cent. Sci. 2015, DOI: 10.1021/acscentsci.5b00279). In one example, the researchers showed how a trimeric copper porphyrin cage holding a C60 molecule unravels on cue when triphenyl phosphine binds copper. Nitschke explained that the phosphine functions as a “chemical fuel” to disrupt the equilibrium of the system, similar to the way protein phosphorylation can dynamically control self-assembly in cells. While the phosphine fuel is being consumed—at a controlled rate dictated by a rhenium catalyst—the system remains disassembled. When the phosphine fuel is used up, the cage reassembles and retraps the C60 inside.Stephen K. Ritter


How To Design A Possible Alzheimer’s Drug

ENGINEERED PROTEIN
Crystal structure of an engineered near-IR bacterial phytochrome fluorescent protein called BphP1-FP.
Credit: Research Collaboratory for Structural Bioinformatics Protein Data Bank
Researchers were able to crystallize and determine the structure of the main body of BphP1-FP (ribbons) bound to a biliverdin chromophore (ball-and-stick structure). Green spheres are water molecules.

The pathway to a treatment for Alzheimer’s disease is littered with drugs that looked promising but ultimately failed. One drug candidate still soldiering on is Merck & Co.’s verubecestat, which is currently in Phase III clinical trials. At Pacifichem, Merck scientist Daniel Wyss reported how scientists developed the clinical candidate. Verubecestat targets β-site amyloid-cleaving enzyme 1, also known as BACE1 or β-secretase. The enzyme is involved in generating amyloid-β, the peptide responsible for the plaques that accumulate in the brains of Alzheimer’s patients. Roughly 10 years ago, scientists considered BACE1 an undruggable target, Wyss told C&EN. To tackle BACE1, researchers at Schering-Plough decided to use fragment-based lead discovery (FBLD)—a relatively new approach to drug discovery at the time. (Schering-Plough later merged with Merck.) In contrast to the classical drug-discovery approach of high-throughput-based screening, in which scientists screen a library of millions of small molecules against a particular target, FBLD tests only thousands of simpler chemical motifs to see if they bind in a target’s active site. “It’s like a puzzle,” Wyss explained. “You take the building block and you try to gradually expand it into nearby binding pockets.”

Wyss’s team initially hit upon an isothiourea as the starting piece for their discovery process. Unfortunately, isothioureas are unstable. X-ray crystallography revealed that the isothiourea moiety was making an extended network of hydrogen bonds in BACE1’s active site with functional groups that were key to the enzyme’s catalytic activity. “Those kinds of interactions were unknown in the field,” Wyss said. The researchers determined that iminopyrimidinones could bind in the same manner as the isothiourea moiety. They then modified the molecule further by adding a fluorinated phenyl ring so it could extend into one of the nearby binding regions in BACE1, where hydrophobic interactions were key. After some classical medicinal chemistry, they arrived at the clinical candidate, verubecestat. The approach, said Daniel Erlanson, the symposium organizer and cofounder of Carmot Therapeutics, a biotech company that focuses on FBLD, “has delivered a molecule with sufficiently good properties that Merck will be able to directly test the amyloid hypothesis for Alzheimer’s disease.” Bethany Halford


A New And Improved Fluorescent Protein

Green fluorescent proteins (GFPs) revolutionized scientists’ ability to monitor happenings inside cells. But the glowing proteins have a serious limitation when it comes to watching biological phenomena in whole, living animals. The wavelengths of light used to excite GFPs are readily absorbed by animal tissue, making it difficult for researchers to peer past the surface of an organism. At Pacifichem, scientists reported a newly engineered near-infrared fluorescent protein that could improve whole-organism imaging of biological processes. The new protein is a modified member of a bacterial class of phytochrome fluorescent proteins, which bind near-IR-absorbing tetrapyrroles called bilins. But the native proteins “are not meant to fluoresce,” explained biophysicist John T. M. Kennis of VU University Amsterdam, who was not involved in the new work. “They are signaling proteins that have evolved to detect near-IR light and give off a signal to the organism to act in certain ways. So these proteins have to be engineered to become fluorescent.” Vladislav Verkhusha and Daria Shcherbakova of Albert Einstein College of Medicine and coworkers have been working on engineering the proteins to absorb and emit light from different stretches of the near-IR spectrum. Recently, the researchers found they could tune the proteins’ spectral properties by changing the amino acid residue to which a bilin called biliverdin attaches to the protein. Their best success to date is a protein they call BphP1-FP (Chem. Biol. 2015, DOI: 10.1016/j.chembiol.2015.10.007). It emits at 669 nm, at the edge of the 650 to 900 nm near-IR spectral region. BphP1-FP has a fluorescence quantum yield, a measure that contributes to the protein’s brightness, of 13%, “which is the highest ever reported for bacterial phytochromes,” Kennis said. He noted that the first reported near-IR bacterial phytochrome fluorescent protein, developed in 2009, had a quantum yield of about 7% and that there had been few improvements on that since then. Stu Borman

Article:

This article has been sent to the following recipient:

0 /1 FREE ARTICLES LEFT THIS MONTH Remaining
Chemistry matters. Join us to get the news you need.