Using High Performance NanoLC and MicroLC for Proteomics Applications
 
 
CEN Webinars: Stronger Bonds

Using High Performance NanoLC and MicroLC
For Proteomics Applications

 

Thursday, September 20, 2012

USA 11:00 a.m. EDT / 10:00 a.m. CDT / 8:00 a.m. PDT / 15:00 GMT

 

Speakers


Christie Hunter
Director
Proteomic Pharma Applications
AB SCIEX



Dr. Nicholas Morrice
Group Leader
The Beatson Institute for Cancer Research


Moderator


Linda Wang
Senior Editor
C&EN
 


 

Overview:


Proteomic workflows using nano-LC MS/MS are relatively robust but often slow relative to small molecule analysis. Using a hybrid PEEKSil/stainless steel electrode (25mm id) for the Duospray/Turboionspray source, we can now obtain similar sensitivity as nano flow LC MS/MS for proteomics workflows performed on a AB SCIEX TripleTOF® 5600 System. This hybrid electrode is totally compatible with capillary LC flow rates of 3-5 ml/min, which results in highly reproducible retention times, narrow peak widths, shorter runtimes and makes it highly attractive for label free quantitative proteomics.

 

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