Database Assisted Capillary Electrophoresis for Analytical Glycomics
Thursday, January 24, 2013
USA 11:00 a.m. EST / 10:00 a.m. CST / 8:00 a.m. PST / 16:00 GMT
Who should attend?
• Development & QC scientists
• Researchers/ R&D Managers
• Laboratory Managers/ Directors / Supervisors
• Laboratory Technicians / Operators
Horvá́th Laboratory of Bioseparation Sciences
University of Debrecen
Capillary electrophoresis (CE) is a rapid and high resolution separation tool for the analysis of complex carbohydrates, also offering full structural elucidation options. In spite of CE being a rather simple separation technique, it's in fact highly adaptive and multi-facetted, enabling various separation modes, simply by changing the background electrolyte. Facing the challenge of the immense structural diversity of carbohydrates, comprising positional and/or linkage isomers, CE is today a priceless tool for their analysis due to its hydrodynamic volume differences based separation principle. Sequential exoglycosidase digestion and analytical/preparative chromatography are providing valuable complementary or even orthogonal information, thus expanding the scope of CE. Recently, assisted by the emerging field of glycoinformatics, the identity of glycan structures can be readily assigned relative to their electrophoretic migration time based glucose unit (GU) values. With the aid of such database, CE now offers reliable, precise and traceable glycan structural elucidation of biotherapeutics and other glycoproteins of biomedical importance.
Participants Will Learn:
• Basics of CE for analysis of glycans
• Importance of analyzing carbohydrates on biotherapeutics
• How CE can be used to help identify complex glycans