Exploring the Proteome and Glycome using Chip-Based nanoLC
 
 
CEN Webinars: Stronger Bonds

Exploring the Proteome and Glycome using Chip-Based nanoLC

 

Thursday, November 15, 2012

USA 11:00 a.m. EST / 10:00 a.m. CST / 8:00 a.m. PST / 16:00 GMT

 

Speakers


David C. Muddiman, Ph.D.
Professor of Chemistry
North Carolina State University


Speakers


Remco Van Soest
Director Product Management
Eksigent


Moderator


Jyllian Kemsley, Ph.D.
Senior Editor
C&EN
 


 

Overview:


Mass-spectrometry has the ability to elucidate new biomarkers and translate them into useful research applications. However, there are numerous challenges that exist that must be overcome including transferring methods from a discovery-based MS platform to a target-based MS platform and maintaining reproducible retention times and separation efficiency for a diverse range of analytes over hundreds to thousands of injections. This presentation will discuss our efforts in global proteomics of several different systems, and ultimately translating those findings into targeted absolute quantification methods. In addition to proteomics, we have developed hydrophobic tagging reagents to increase the electrospray response of N-linked glycans which has the major added benefit of being able to incorporate a stable isotope label for use in relative quantification experiments. These studies have greatly benefited from the high efficiency separations and reproducibility of the Eksigent cHiPLCĀ® system.

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