Overview:

Mass-spectrometry has the ability to elucidate new biomarkers and translate them into useful research applications. However, there are numerous challenges that exist that must be overcome including transferring methods from a discovery-based MS platform to a target-based MS platform and maintaining reproducible retention times and separation efficiency for a diverse range of analytes over hundreds to thousands of injections. This presentation will discuss our efforts in global proteomics of several different systems, and ultimately translating those findings into targeted absolute quantification methods. In addition to proteomics, we have developed hydrophobic tagging reagents to increase the electrospray response of N-linked glycans which has the major added benefit of being able to incorporate a stable isotope label for use in relative quantification experiments. These studies have greatly benefited from the high efficiency separations and reproducibility of the Eksigent cHiPLC® system.