Open 10 Doors at Once: Sample Multiplexing with 10-plex Tandem Mass Tags (TMT) Provides a New Perspective on any Biology

March 16, 2017

8:00 a.m. PDT / 11:00 a.m. EDT / 16:00 BST / 17:00 CEST

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Overview

 

The ability to perform accurate protein quantification at low levels lets scientists unravel the complexity of protein interactions, and track protein abundance changes in a wide variety of samples and applications.

The proteome is complex and dynamic and innovative tools are needed to study the complexities of biology. In fact, the full measure of proteome changes can rarely be encapsulated in a single time point representing a snapshot of the dynamics. Sample multiplexing using Tandem Mass Tag (TMT) when combined with an appropriate Thermo Scientific Orbitrap HRAM MS enables scientists to ask real questions of their samples and obtain a meaningful description of how protein expression changes with disease, with the progression of time, and even within different parts of the cell. TMT workflows enable more than simple multiplexed quantitation; they open the door to defining whole new sets of questions.

In this webinar, case studies will be highlighted from the Gygi lab and ThermoFisher Center for Multiplexed Proteomics at HMS demonstrating the robustness of the technique, and more importantly, how to apply these workflows to a variety of analyses to answer a breadth of biological questions.

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Key Learning Objectives

 

• Understand basic concept of multiplexed proteomics with isotope labeled tandem mass tags

• How to apply TMT workflows to answer diverse biological questions

Who Should Attend:

 

• Researchers performing large, disease-related profiling studies

• Protein biochemists and biologists studying protein regulation and function in cellular signaling

• Protein mass spectrometrists interested in improving protein quantitation, throughput and results

 

Speakers

 
Steven Gygi, Ph.D.,
Professor Cell Biology,
Harvard Medical School
Edward Huttlin, Ph.D.,
Department of Cell Biology,
Harvard Medical School
 
 

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