2-Part Webinar Series: Opening Doors to a Whole New View of Biology with TMT Multiplexing Workflows

Protein Quantitation Part 2: LC-MS - Practical Guidelines for Robust, Accurate, and Meaningful Quantitative Proteomics

November 9, 2016

8:00 a.m. PST / 11:00 a.m. EST / 16:00 GMT / 17:00 CET

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Overview

 

The proteome is complex and dynamic. You need innovative tools to study the complexities of biology. Instead of a single data point, snapshot or limited capabilities of other reagents, monitor the real changes at work. Multiplexed tandem mass tag (TMT) solutions enable you to ask real questions of your sample and obtain a meaningful description of how protein expression changes with disease, with the progression of time, and even within different parts of the cell. TMT workflows enable more than simple multiplexed quantitation; it enables solving life's questions.

So go beyond snapshots and analyze global protein dynamics more deeply.

We're pleased to bring you this 2-part webinar series, where attendees can expect to gain practical knowledge and advice across the entire multiplexed quantitative proteomics workflow – from good sample preparation practices, to LCMS optimization, to obtaining biological meaning from MS results with advanced software.

Protein Quantitation Part 2: LC-MS - Practical Guidelines for Robust, Accurate, and Meaningful Quantitative Proteomics
The development of the SPS MS3 workflow exclusive to Thermo Scientific™ Tribrid mass spectrometers has taken this workflow to the next level, bringing unmatched accuracy and precision to biological research in the field of quantitative proteomics. In the first half of this webinar, we will focus on achieving sensitive, accurate and high-protein-coverage relative quantitation with multiplexed TMT technology for conventional proteomics and phosphoproteomics applications. Discussion will center on instrumental parameters and separation conditions that will enable researchers to be successful in this regard.

Multiplexing workflows also rely on robust software algorithms for the consistent retrieval and interpretation of reporter ion signals to measure relative protein abundance across quantification channels. The newly introduced study management tool in Thermo Scientific™ Proteome Discoverer™ software enables users to set up and evaluate results from advanced multiplexed experiments. The capacity of the software to process large data sets has been considerably enhanced and new capabilities, such as peptide and protein probability/FDR calculation, and persistent reports, have been added. TMT-based reporter-ion quantification steps, associated with obtaining reproducible protein quantification results will be reviewed in the second half of this webinar. Finally we will conclude the webinar with a discussion on reducing complexity and adding biological meaning to proteomics experiments using powerful cloud tools.

Don't forget to register for Part 1 of this webinar series
Protein Quantitation Part 1: Mass Spectrometry Sample Prep for New and Experienced Users

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Participants Will Learn:

 

• MS sample preparation methods to reduce proteome complexity

• Techniques and reagents to improve sample prep reproducibility for protein quantitation workflows

• Instrument parameters and separation conditions for a successful SPS MS3experiment

• Methods for sensitive and accurate multiplexed quantitative phosphoproteomic applications

Who Should Attend:

 

• Protein biochemists and biologists studying protein regulation and function in cellular signaling

• Protein mass spectrometrists interested in improving protein quantitation, throughput and results

 

Speakers

 
Xiaoyue, Ph.D.,
Senior Strategic Marketing Specialist,
Life Sciences Mass Spectometry
Thermo Fisher Scientific
David Horn, Ph.D.,
Product Manager,
Proteome Discoverer,
Life Sciences Mass Spectometry
Thermo Fisher Scientific
Devin Drew, Ph.D.,
Product Manager,
Omics Software,
Life Sciences Mass Spectrometry,
Thermo Fisher Scientific
 

Register Now