Automated and High Throughput Dynamic Light Scattering Application Examples
Thursday, November 8, 2012
USA 11:00 a.m. EDT / 10:00 a.m. CDT / 8:00 a.m. PDT / 16:00 GMT
Who should attend?
• Bench Chemists
• Laboratory Managers/Directors/Supervisors
• Protein Scientists
• Formulation scientists with 10s, 100s, or many 1000s of samples and sample conditions, for which any of: stability, viscosity, turbidity, or solubility are parameters of interest
Michael Larkin, Ph.D.
Director of Advanced Projects
Wyatt Technology Corporation
Stephen K. Ritter, Ph.D.
Dynamic light scattering (DLS) measures the Brownian motion of molecules and particles in solution, from which size and size distributions may be determined. Automation of DLS measurements with low sample volume requirements has enabled new applications, which were prohibitive without automation. In this webinar we will briefly introduce the DLS method, and present both traditional and newer applications of automated/high throughput DLS, including 1) formulation optimization for protein crystallization, 2) determination of the solubility point of small molecules for entire small molecule libraries (e.g., minimizing false positives and negatives when screening), 3) making automated turbidity measurements, 4) high throughput/low volume viscosity determinations (a breakthrough for multi-axis formulation work), and 5) degree of molecular attraction/repulsion for formulation development.
Participants Will Learn:
• Brief review of dynamic light scattering
• How automated, high throughput, low volume dynamic light scattering may be used to:
- determine the size and size distribution of particles and molecules in solution
- measure sample viscosity, turbidity, and solubility
- provide formulation scientists with the degree of attraction or repulsion between molecules or particles in solution