Automated Measurements of Electrophoretic Mobility (Zeta Potential) for Proteins, Biopolymers and Nanoparticles
 
 
CEN Webinars: Stronger Bonds

Automated Measurements of Electrophoretic Mobility
(Zeta Potential) for Proteins, Biopolymers and Nanoparticles

 

December 8, 2011

USA 11:00 a.m. EST / 10:00 a.m. CST / 8:00 a.m. PST / 16:00 GMT

 

Who should attend?


• Scientists interested in characterizing macromolecular or particle charge in solution.

• Researchers who wish to automate mobility measurements and increase the throughput of their research and experiments.

• Researchers who want to measure electrophoretic mobility in high-conductivity samples.

• Formulation scientists.

• Researchers who want to learn about a state-of-the-art analytical technique for efficiently characterizing proteins, particles and polymers in solution.

Speaker


Steven Trainoff, Ph.D.
Director of Engineering
Wyatt Technology
Corporation



Moderator


Stuart Borman, Ph.D.
Senior Editor
C&EN


This webinar will present a few exciting developments associated with advancements in making measurements of electrophoretic mobility (zeta potential). While measuring electrophoretic mobility in solutions has been a common technique for decades, advancements in solid-state electronics and electro-optics now enables this technique to be applied to proteins and anti-bodies whose delicate nature precluded them from this characterization.

Whereas before, these measurements were tediously performed by sequential, manual operation, zeta potential can now, for the first time, be automated using an auto-injector. Moreover, the challenging electrolysis problem faced by researchers interested in measuring sample mobility in high-salt solutions will be shown to be conquered at last. Now samples with high ionicity (at or above physiological saline) can be measured easily. Data of protein mobility measurements will be presented to elucidate the utility of these new techniques.

Topics Covered/Participants Will Learn:


• What kinds of samples can be measured and characterized by electrophoretic mobility

• How zeta potential can be combined with an HPLC pump and autosampler to enable automated, unattended measurements of electrophoretic mobility and hydrodynamic radius

• How high-salt samples can be measured without electrolysis

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