Amphotericin B Mystery Solved | March 21, 2011 Issue - Vol. 89 Issue 12 | Chemical & Engineering News
Volume 89 Issue 12 | p. 51
Issue Date: March 21, 2011

Amphotericin B Mystery Solved

Decades-long question about Antifungal Agent’s Mechanism is answered
Department: Science & Technology
Keywords: amphotericin B, antifungal agents, ion channels

Chemical synthesis has resolved the long-standing uncertainty about how the potent but highly toxic antifungal natural product amphotericin B works. The findings could lead to rational design of analogs with fewer side effects than the natural compound.

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Credit: Proc. Natl. Acad. Sci. USA
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Credit: Proc. Natl. Acad. Sci. USA

Amphotericin B, a cyclic polyene macrolide, is used to treat progressive and life-threatening fungal infections, but it has serious side effects, including potentially fatal cardiac or cardiopulmonary arrest. Scientists have known that its activity is due to formation of ion channels, but despite decades of research, they have not been able to determine how the channels assemble.

Three hypotheses to explain how amphotericin B molecules gang up to create cell-killing ion channels in fungal cell membranes differ in the molecular interactions that drive ion-channel formation. In one model, mycosamine and carboxylate groups on neighboring amphotericin B molecules form noncovalent bonds. In the second model, mycosamine and/or carboxylate groups form polar interactions with head groups of membrane phospholipids. In the third, the mycosamine and/or carboxylate groups bind to membrane-embedded sterols such as ergosterol and cholesterol.

This last model is the correct one, Martin D. Burke and coworkers at the University of Illinois, Urbana-Champaign, report in a recent paper (Proc. Natl. Acad. Sci. USA, DOI: 10.1073/pnas.1015023108). Burke’s team came to this conclusion by synthesizing and testing derivatives of amphotericin B that lack the mycosamine group, the carboxylate group, or both. They also found that amphotericin B requires the mycosamine group—but not the carboxylate—for its molecules to interact with ergosterol or cholesterol and assemble into ion channels.

Maciej Baginski, an expert on the mechanism of action of antifungal polyene macrolide antibiotics at Gdansk University of Technology, in Poland, comments that the finding “that amphotericin B molecules without the carboxyl group can still form channels and exhibit antifungal activity is a very new and rather surprising observation from the perspective of all 50 years of studies on the molecular mechanism of amphotericin B action.”

The findings “will help focus efforts toward the rational optimization of the therapeutic index of this clinically vital but also highly toxic antimycotic,” Burke and coworkers note in their paper. “These studies vividly demonstrate the power of synthesis-enabled functional group deletions to illuminate highly elusive fundamental underpinnings of small-molecule function.”

 
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