CEN Webinars: Stronger Bonds
Purity Analysis of Bispecific Antibodies by Affinity Capillary Electrophoresis
Wednesday September 17th 2014
8:00a.m. PDT / 11:00a.m. EDT / 16:00 BST
SPEAKER
Speaker
Jiann Kae "Henry" Luo, PhD.,
Senior Staff Scientist,
Protein Biochemistry,
Regeneron Pharmaceuticals
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MODERATOR
Moderator
Stu Borman,
Senior Correspondent,
C&EN
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OVERVIEW

Monoclonal antibodies represent an important class of therapeutics for various diseases. There is a growing interest in increasing the versatility of monoclonal antibodies, with one approach being the design and generation of bispecific antibodies (BsAbs). A BsAb possesses two different antigen binding sites, which can be useful for targeting strategies or increasing potency. One class of bispecific antibody has the format of a conventional antibody, with two distinct heavy chains of different binding specificities. Co-expression of these components will typically also result in formation of undesirable homodimeric monospecific antibodies, which must then be removed in the purification process. In order to assess the efficiency of the purification procedure, a capillary zone electrophoresis (CZE) assay was developed to specifically identify small amounts of the homodimers in the purified bispecific samples. The CZE assay takes advantage of a high resolution separation method first described by He and co-workers [1], and incorporates the use of immunoaffinity ligands to provide identity to the peaks separated by CE. This CZE method offers high resolution, short experimental times and broad applicability to many different BsAbs.

[1] He, Y., Lacher, N.A., Hou, W., Wang, Q., Isele, C., Starkey, J. and Ruesch, M. (2010)Anal. Chem. 82: 3222-30.

KEY LEARNING OBJECTIVES:

- The use of CZE in analytical characterization of protein therapeutics

- Importance of bispecific antibody analysis

- Application of Partial Fill Affinity CE for purity analysis

WHO SHOULD ATTEND
  • • Analytical Development Scientists
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  • • Researchers/ R&D Managers
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  • • Laboratory Managers/ Directors / Supervisors
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  • • Laboratory Technicians / Operators
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