Untitled Document

Micro-RNA Nanoparticles for Gene Silencing: Measuring Size and Zeta Potential with Light Scattering Technologies

June 8, 2016

8:00 a.m. PDT / 11:00 a.m. EDT / 16:00 BST / 17:00 CEST

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Accurately measuring the stability of biological molecules is a critically important step in the development of novel biologic-based therapeutics. Since nanoparticle properties such as size and zeta potential may influence particle uptake by target cells and/or tissues in vivo, light scattering technologies represent an important tool to investigate the physicochemical properties of miRNA-delivery systems. Here we demonstrate that the Litesizer™ 500 returns rapid and precise results despite very low sample concentration and volume. We used dynamic light scattering (DLS) for particle size measurement and a novel, patented electrophoretic light scattering technology called cmPALS for zeta potential determination.

Continuously Monitored Phase Analysis Light Scattering (cmPALS) enables faster and more reproducible zeta potential analysis. Specifically, the technology includes an additional monitor that measures and removes any non-linearities in the frequency modulator and thus enables faster measurements at lower voltages with improved reproducibility.

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Key Learning Objectives


• Sample Preparation of self-assembled RNA nanoparticles

• Benefits of the new cmPALS technology for particle analysis

• Benefits of the new Omega Cuvette for Zeta Potential Analysis

• Advantages in using DLS and ELS to optimize biological formulations

Who Should Attend:


• Researchers/ R&D Mangagers

• Laboratory Managers/ Directors / Supervisors

• Laboratory Technicians / Operators



Andreas Zimmer, Ph.D.,
Betty Petrillo, Ph.D.,
International Product Manager,
Anton Paar


Alexandra Taylor,
Associate Editor,

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