CEN Webinars: Stronger Bonds
QTOF Instrumentation for Quantitative Proteomics
Wednesday November 4th 2015
USA 11:00am EST, 8:00am PST, 16:00 GMT, 17:00 CET
SPEAKERS
Speaker
Scarlet Beck,
PhD Student,
Max Planck Institute of Biochemistry Martinsried,
Germany
 
Speaker
Florian Meier,,
PhD Student,
Max Planck Institute of Biochemistry Martinsried,
Germany
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MODERATOR
Moderator
Jessica Morrison,
Assistant Editor,
C&EN
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OVERVIEW

Hybrid quadrupole time-of-flight (QTOF) mass spectrometry is one of the two major principles used in proteomics. Although based on simple fundamentals, it has over the last decades greatly evolved in terms of achievable resolution, mass accuracy and dynamic range. Recently, we established the impact II, a benchtop QTOF instrument, as a powerful instrument for shotgun proteomics of thousands of proteins in biological samples (Beck et al., MCP 2015).

In this webinar, examples of how the technology was applied for label-free quantification in biological case studies will be described. We will furthermore discuss critical factors for the success of these experiments, starting from sample preparation and liquid chromatography to data analysis with MaxQuant.

KEY LEARNING OBJECTIVES:

- Learn fundamentals of QTOF instrumentation and nanoflow liquid chromatography with focus on proteomics applications

- Get to know specific challenges in quantitative proteomics

- Gain insight into method development and data analysis with MaxQuant

- Hear specific examples of how these approaches have been applied to identify and quantify proteomes

WHO SHOULD ATTEND
  • • MS practitioners
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  • • Researchers/ R&D Managers
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  • • Laboratory Managers / Directors / Supervisors
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  • • Laboratory Technicians / Operators
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