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Improve Your Monoclonal Antibody Separations by Leveraging The Advantages of Fused-Core® Columns



  July 25, 2018

  USA 11:00 a.m. EDT, 8:00 a.m. PDT, 16:00 BST




With the increasing emphasis on biotherapeutics in the pharmaceutical industry, many researchers have goals of obtaining sharp, well-resolved peaks for their LC analyses of larger, complex biomolecules, such as monoclonal antibodies (mAbs), biosimilars, and fusion proteins. In this webinar, we will describe several methods to improve biomolecule separations using columns packed with Fused-Core particles. The Fused-Core particle design, with its solid silica core and thin porous shell, enables fast, rugged separations for biomolecules of various sizes, including peptides, proteins, as well as large monoclonal antibodies, and their fragments and variants.

Careful selection and adjustment of parameters, such as optimal pore size, bonded phase, gradient conditions, mobile phase modifier and additive, and column temperature, all contribute to significantly improved analysis of biotherapeutics. The importance of each of these parameters will be discussed, along with pertinent examples. In addition, to highlight the advantages of Fused-Core columns, we will compare separations using Fused-Core columns with those obtained using both totally porous particle columns and other superficially porous particle columns.

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Key Learning Objectives

  • Understand the advantages of Fused-Core particles compared to fully porous particles for monoclonal antibody separations
  • Learn how to adjust different parameters of your separation to improve your analysis of monoclonal antibodies
  • Review examples that demonstrate that utilizing large pore size and Fused-Core particles improve monoclonal antibody separations

Who Should Attend

  • Method developers for UHPLC/UPLC and HPLC methods for biomolecules in pharmaceutical, chemical, clinical, environmental, agrichemical, university and governmental laboratories
  • LC Chromatographers looking to separate monoclonal antibodies (mAbs)


Stephanie A. Schuster, Ph.D.,
Application and Quality Manager
Advanced Materials Technology, Inc.


Alane Lim,
Contributing Editor,
C&EN Media Group