Using Novel Sample Preparation Strategies
and High Resolution/Accurate Mass Spectrometry
8:00 a.m. PDT / 11:00 a.m. EDT / 15:00 GMT
Department of Chemistry,
Department of Biochemistry and Molecular Biology
Michigan State University
There is an increasing recognition of the role that aberrant lipid metabolism can play in the onset and progression of several human diseases, including cancer, diabetes and diabetic complications. Therefore, there is a need for the development of robust, sensitive and rapid analytical strategies to comprehensively profile the diverse range of individual molecular lipid species that can be present within a complex lipidomic mixture, as well as to quantitatively characterize their changes.
In this presentation, the development and application of an improved analytical strategy for comprehensive 'shotgun' lipidome analysis will be described, involving a monophasic extraction protocol for the simultaneous analysis of both highly polar and nonpolar lipid species, coupled with functional group selective derivatization reactions and direct infusion nano-ESI-high resolution/accurate mass spectrometry (MS) and 'targeted' MS/MS.
The application of these strategies are expected to provide critical insights for the design of subsequent studies aimed at elucidating the functional role of specific lipid metabolism pathways in the pathogenesis of these diseases, as well as to identify specific lipid(s) that can serve as effective diagnostic or prognostic disease biomarkers.
- Understanding improved analytical strategies for lipidome analysis
- Learn how the application of these strategies can provide insight on study design for better understanding disease pathogenesis
- Learn how to identify lipids that can serve as effective diagnostic disease biomarkers