Developing Cross-linking Mass Spectrometry to Define Protein Complexes

Developing Cross-linking Mass Spectrometry to Define Protein Complexes

March 30, 2017

8:00 a.m. PDT / 11:00 a.m. EDT / 16:00 BST / 17:00 CEST

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Cross-linking mass spectrometry (XL-MS) has recently become a powerful strategy for delineating protein-protein interactions (PPIs) and analyzing structures of large protein assemblies within large multi-subunit protein complexes. XL-MS has been proven to be effective in elucidating molecular details of PPIs at the peptide resolution, providing a complementary set of structural data that can be utilized to refine existing complex structures or direct de novo modeling of unknown protein structures.

In this webinar, we will describe the development of a novel class of MS-cleavable cross-linkers shown to be effective in facilitating the unambiguous identification of cross-linked peptides, as the resulting cross-linked products can be identified based on their characteristic and simplified fragmentation behavior during MS analysis with great utility for in vivo studies.

The potential of this technology is enormous, and with continued advances in mass spectrometry instrumentation, this new XL-MS strategy has been shown to map in vivo PPIs from mammalian cells at both the proteome scale and the targeted protein complex level.

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Key Learning Objectives


• Learn about why to fully understand protein functions, the proteins involved should be studied in the context of their interactions

• Benefits of the new Omega Cuvette for Zeta Potential Analysis

• Learn about how cross linking mass spectrometry has emerged as powerful and essential tool for identifying transient protein interactions and refining protein structures

• Learn about the challenges of studying cross linking and the evolution of MS equipment and reagents to meet this need

Who Should Attend:


• Researchers studying protein functions

• Researchers performing protein structural analysis using crosslinkers

• Protein biochemists and biologists performing protein-protein interaction studies



Lan Huang, Ph.D.,
Professor Physiology & Biophysics in School of Medicine,
University of California, Irvine


Ann Thayer,
Senior Correspondent,

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