CEN Webinars: Stronger Bonds

Improving your Analysis by Design


Tuesday, May 21, 2013

USA 11:00 a.m. EDT / 8:00 a.m. PDT / 16:00 BST/ 17:00 CEDT


Who should attend?

• All practicing chromatography method developers

• Analytical scientists who are interested in learning how science can be applied to the art of chromatography


Tony Edge
R&D Principal
Chromatography Consumables Division,
Thermo Scientific


Stu Borman
Senior Correspondent


The development of a chromatographic method for the separation and quantification of a series of components is done routinely in many laboratories. It is often seen as an art and not as a scientific practice. There is a perception that it requires many years to learn the subtleties of column selection and even longer to understand the nuances of mobile phase and pH determination to optimize a separation.

This seminar will begin by looking at what key physiochemical properties of the molecules to be separated are important and how this information can be readily obtained and then used to develop a separation.

The approach will be to combine characterization tests (popularized by Tanaka as a way of fingerprinting some of the key mechanisms that can drive a separation process), with the information gathered from the molecule to predict a separation.

The choice of mobile phase can be critical in optimizing a separation and several key components will be considered including:

• The effect that pH can have on the resolution of critical pairs and as well as peak shape.

• How buffer selection can have an important effect on the robustness of the assay.

• The choice of optimization of organic solvent will be investigated.

Examples will be given throughout the seminar to demonstrate how this approach is effective in providing a quick and robust approach to developing a separation.

Key Learning Objectives:

• How to optimize column selection

• How to ensure the correct mobile phase is chosen

• How to do some basic troubleshooting of an assay in the method development stage

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