Multiplexed Quantitative Proteome and Ubiquitylome Analysis Utilizing nLC-MS/MS and Tandem Mass Tags
CEN Webinars: Stronger Bonds
Multiplexed Quantitative Proteome and Ubiquitylome Analysis Utilizing nLC-MS/MS and Tandem Mass Tags
Tuesday June 23rd 2015
USA 11:00am EDT, 8:00 am PDT, 16:00 BST
SPEAKER
Speaker
Christopher Michael Rose,
Postdoctoral Research Fellow,
Harvard Medical School
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MODERATOR
Moderator
Stu Borman,
Senior Correspondent,
C&EN
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OVERVIEW

Large-scale, multiplexed quantitative proteomics experiments have recently become more accessible due to advances in both mass spectrometry instrumentation and reagents. Today, by utilizing isobaric labels (e.g., Tandem Mass Tags), >8,000 proteins can be routinely quantified across 10 experimental samples in 1.5 days of analysis. In this webinar both sample preparation and instrument methods used to collect large scale quantitative proteomic data will be presented. Specifically, I will highlight an MS3 instrument method (i.e., synchronous precursor selection MS3, SPS-MS3) that removes precursor interference and alleviates compression of measured ratios. Lastly, I will discuss recent developments in the analysis of ubiquitylomes using multiplexed analysis, where >15,000 sites of ubiquitylation were simultaneously quantified across 10 quantitative channels.

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