2-Part Webinar Series: Opening Doors to a Whole New View of Biology with TMT Multiplexing Workflows

Protein Quantitation Part 1: Mass Spectrometry Sample Prep for New and Experienced Users

August 30, 2016

8:00 a.m. PDT / 11:00 a.m. EDT / 16:00 BST / 17:00 CEST

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Overview

 

The proteome is complex and dynamic. You need innovative tools to study the complexities of biology. Instead of a single data point, snapshot or limited capabilities of other reagents, monitor the real changes at work. Multiplexed tandem mass tag (TMT) solutions enable you to ask real questions of your sample and obtain a meaningful description of how protein expression changes with disease, with the progression of time, and even within different parts of the cell. TMT workflows enable more than simple multiplexed quantitation; it enables solving life's questions.

So go beyond snapshots and analyze global protein dynamics more deeply.

We're pleased to bring you this 2-part webinar series, where attendees can expect to gain practical knowledge and advice across the entire multiplexed quantitative proteomics workflow – from good sample preparation practices, to LCMS optimization, to obtaining biological meaning from MS results with advanced software.

Protein Quantitation Part 1: Mass Spectrometry Sample Prep for New and Experienced Users
While mass spectrometers have improved rapidly over the past few decades, results have remained largely dependent on the quality of the sample, and, by extension, sample preparation that is undertaken for analysis. Part 1 of this webinar will describe the rationale, considerations, and general techniques used to prepare samples for proteomic MS analysis. Additionally, we will also provide an overview of methods for reducing sample complexity and tools for improving proteomics sample preparation reproducibility.

Don't forget to register for Part 2 of this webinar series
Protein Quantitation Part 2: LC-MS - Practical Guidelines for Robust, Accurate, and Meaningful Quantitative Proteomics

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Particpants Will Learn:

 

• MS sample preparation methods to reduce proteome complexity

• Techniques and reagents to improve sample prep reproducibility for protein quantitation workflows

• Instrument parameters and separation conditions for a successful SPS MS3 experiment

• Methods for sensitive and accurate multiplexed quantitative phosphoproteomic applications

Who Should Attend:

 

• Protein biochemists and biologists studying protein regulation and function in cellular signaling

• Protein mass spectrometrists interested in improving protein quantitation, throughput and results

 

Speaker

 
Ryan Bomgarden, Ph.D.,
Sr. Staff Scientist,
Thermo Fisher Scientific
 

Moderator

 
Britt Erikson,
Senior Editor,
C&EN
 

Register Now