in Tissues and Cell Lines
11:00 a.m. EDT, 10:00 a.m. CDT, 8:00 a.m. PDT, 15:00 GMT
Thermo Fisher Scientific
Despite the success of mass spectrometry-based analysis of protein expression, the more fundamental question of protein turnover remains a challenge.
The proteome is extremely dynamic and exists in constant flux. Most studies present one particular state of proteome at a given time with given conditions and do not describe the level of proteinsynthesis and degradation. Recent attempts to study protein turnover, based on stable isotope labeling, use different approaches and different algorithms for data processing. Here we perform a comparative analysis of rates of protein turnover from these different approaches from a variety of tissues and cell lines using the Thermo Scientific™ ProteinCenter™ software.
Understand the challenges of complex comparison studies of protein turnover derived from experimental data, available databases and literature