Finding the Balance: Setting Up Data Independent Acquisition on a Q Exactive HF MS Using Spectronaut and Application to a Hepatotoxicity Study
CEN Webinars: Stronger Bonds
Finding the Balance: Setting Up Data Independent Acquisition on a Q Exactive HF MS Using Spectronaut and Application to a Hepatotoxicity Study
Thursday October 30th 2014
8:00 a.m. PDT / 11:00 a.m. EDT / 16:00 BST
SPEAKERS
Speaker
Dr. Lukas Reiter,
Head of Research & Development
BiognoSYS AG
 
Speaker
Dr. Yue Xuan,
Product Specialist FT-MS
Thermo Fisher Scientific
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MODERATOR
Moderator
Linda Wang,
Senior Editor,
C&EN
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OVERVIEW

This webinar, in two parts, will cover the development of an optimized Data Independent Acquisition (DIA) workflow for a proteomics study using the Thermo Scientific™ Q Exactive™ HF mass spectrometer and Spectronaut™ software.

In the first part, Yue Xuan will introduce the Q Exactive HF hybrid quadrupole-Orbitrap mass spectrometer featuring an ultra-high- field Orbitrap analyzer, which doubles its speed and resolution. The benefits of fast scan speed, high mass accuracy, and excellent HCD MS/MS spectra quality of QE HF MS for DIA applications are reviewed along with a demonstration of the workflow strategy using data from analysis of various samples.

In the second part, Lukas Reiter will present DIA for protein profiling of human 3D liver microtissues on a Q Exactive HF MS and using the Spectronaut™ software. The microtissues were exposed to biologically relevant concentrations of Acetaminophen and measured in triplicates. With the instrumentation and software advancements it became feasible to robustly profile thousands of proteins across dozens of conditions with reasonable time, cost and very limited starting material.

KEY LEARNING OBJECTIVES:

- The prerequisites, including both hardware and software, for the data independent acquisition (DIA) current workflow

- The DIA workflows based on Q Exactive HF MS platform

- High Resolution Accurate Mass information for both MS/MS and MS to confident identify and quantify samples

- A study of DIA protein profiling of human 3D liver microtissues on a Q Exactive HF MS and using the Spectronaut™ software

WHO SHOULD ATTEND
  • • Proteomics Scientists
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  • • Mass Spectrometrists
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  • • Core Lab Researches & Directors
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