CEN Webinars: Stronger Bonds
UPLC-ESI-TOF MS based Metabolite Profiling
of the Antioxidative Food Supplement Garcinia buchananii
Wednesday December 9th 2015
USA 11:00am EST, 8:00am PST, 16:00 GMT, 17:00 CET
SPEAKERS
Speaker
Dr Timo Stark, Ph. D,,
Senior Scientist and Head of LC-MS,
Technical University of Munich (TUM)
 
Speaker
Dr Robert Tonge, Ph. D,
Principal Product Manager,
OMICS Informatics,
Waters Corporation
Spacer
MODERATOR
Moderator
Matt Davenport,
Associate Editor,
C&EN
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OVERVIEW

In this webinar we review this analyses and introduce Progenesis QI software and illustrate its use for the quantification and identification of compounds in the different preparations of the antioxidant food supplement Garcinia buchananii. Comparative antioxidative analyses of aqueous ethanolic extracts from leaf, root, and stem of Garcinia buchananii revealed high activity of all three organs. To investigate the metabolites composition of the different parts of G. buchananii, an untargeted metabolomics approach using UPLC-ESI-TOF MS with simultaneous acquisition of low- and high-collision energy mass spectra (MSe) was performed. Unsupervised statistics (PCA) highlighted clear differences in the metabolomes of the three organs. OPLS-DA revealed (2R,3S,2''R,3''R)-GB-1, (2R,3S)-morelloflavone, and (2R,3S)-volkensiflavone as the most decisive marker compounds discriminating leaf from root and stem extract...

KEY LEARNING OBJECTIVES:

- Introduction to food antioxidants

- How to prepare plant extracts for LC-MS analysis

- How to perform UPLC ESI-TOF MS on plant extracts

- How to use Progenesis QI for compound quantification and identification

- How to use PCA to find biomarkers

WHO SHOULD ATTEND
  • • Metabolomics scientists
  •  
  • • Metabolomics managers / Lab Directors / Supervisors
  •  
  • • Laboratory Managers / Directors / Supervisors
  •  
  • • Plant research scientists
  •  
  • • Food research scientists
  •  
  • • Metabolomics informaticians
  •  
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