This webinar will show how complex nanosuspensions, consisting of macromolecules and nanoparticles with sizes 1-1000 nm, can be characterized with high resolution using field-flow fractionation (FFF) coupled to a multi-angle light scattering (MALS) detector. In the life sciences and medicinal fields, FFF-MALS is beneficial for characterizing and isolating gene vectors, liposomal drug formulations, protein aggregates and exosomal biomarkers, but it is also useful in environmental and industrial applications such as lignocellulose, starch, latex paints and effluent or soil particulates.
The principle of separation by Flow-FFF and determination of size using MALS is presented. The FFF 2.0 workflow and novel software platform are demonstrated, from initial simulations and rationalized method refinement to the final, fully-optimized method with data analysis. In addition, the new method of determining electrophoretic mobility by Electrical/Asymmetric Flow-FFF (EAF4) is introduced, with examples to include particles and protein.
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Key Learning Objectives
The principle of FFF and light scattering
How a separation method for complex samples can be developed
The principle of electrical Flow-FFF and how it is used to determine charge and charge distribution
Who Should Attend
Scientists in the fields of nanomedicine and extracellular-vesicle biomarker discovery
Developers of nanoparticle-based drug formulations
Researchers and lab managers in the fields of environmental, food and polymer science who characterize complex sub-micron particle suspensions
Christoph Johann, Ph.D.,
Managing Director, Wyatt Technology Europe GmbH