If you have an ACS member number, please enter it here so we can link this account to your membership. (optional)

ACS values your privacy. By submitting your information, you are gaining access to C&EN and subscribing to our weekly newsletter. We use the information you provide to make your reading experience better, and we will never sell your data to third party members.


_____ Brought to you by Wyatt Technologies _____

Field-Flow Fractionation 2.0: Obtaining high resolution distributions of nanoparticle size and zeta potential, with less time, less effort and more confidence



  July 24, 2018

  8:00 a.m. PDT / 11:00 a.m. EDT / 16:00 BST / 17:00 CEST




This webinar will show how complex nanosuspensions, consisting of macromolecules and nanoparticles with sizes 1-1000 nm, can be characterized with high resolution using field-flow fractionation (FFF) coupled to a multi-angle light scattering (MALS) detector. In the life sciences and medicinal fields, FFF-MALS is beneficial for characterizing and isolating gene vectors, liposomal drug formulations, protein aggregates and exosomal biomarkers, but it is also useful in environmental and industrial applications such as lignocellulose, starch, latex paints and effluent or soil particulates.

The principle of separation by Flow-FFF and determination of size using MALS is presented. The FFF 2.0 workflow and novel software platform are demonstrated, from initial simulations and rationalized method refinement to the final, fully-optimized method with data analysis. In addition, the new method of determining electrophoretic mobility by Electrical/Asymmetric Flow-FFF (EAF4) is introduced, with examples to include particles and protein.

Brought to you by:


Key Learning Objectives

  • The principle of FFF and light scattering
  • How a separation method for complex samples can be developed
  • The principle of electrical Flow-FFF and how it is used to determine charge and charge distribution

Who Should Attend

  • Scientists in the fields of nanomedicine and extracellular-vesicle biomarker discovery
  • Developers of nanoparticle-based drug formulations
  • Researchers and lab managers in the fields of environmental, food and polymer science who characterize complex sub-micron particle suspensions


Christoph Johann, Ph.D.,
Managing Director,
Wyatt Technology Europe GmbH


Linda Wang
Senior Editor,