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Separation and Characterization of Macromolecular and Supramolecular Structures by Light Scattering
February 26, 2019
8:00 a.m. PST / 11:00 a.m. EST / 16:00 GMT / 17:00 CET
Comprehensive characterization of complex macromolecular structures is challenging since no single analytical technique provides adequate information regarding all the different distributions. Hyphenated techniques, that couple separation by asymmetric-flow field-flow fractionation (AF4) or size-exclusion chromatography (SEC) with a multi-detection system, do provide detailed insight into such structures. A versatile online multi-detection system includes multi-angle light-scattering (MALS), dynamic light scattering (DLS), ultraviolet (UV) and refractive-index (RI) detectors.
This webinar explores the application of SEC-MALS-DLS-UV-RI and AF4-MALS-DLS-UV-RI to understanding the molar mass, composition, size, distributions and structure of hybrid polymers and supramolecular structures. Examples include star copolymers, protein-poly(ethylene glycol) conjugates, large unilamellar vesicles, lipid droplets and exosomes.
Brought to you by:
- How size, size distributions and structures are determined by MALS and DLS combined with size-based separation
- Capabilities and limitations of characterization by AF4/SEC-MALS-DLS-UV-RI
- How AF4/SEC-MALS-DLS-UV-RI are applied to specific macromolecular and supramolecular characterization tasks
- Scientists interested in detailed physico-chemical characterization of bionanoparticles, branched or hybrid polymers and related entities.
- Lab managers seeking to explore new technologies for macromolecular and nanoparticle characterization.
Head, Dept. of Polymer Chemistry and Technology,
National Institute of Chemistry, Slovenia