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Separation and Characterization of Macromolecular and Supramolecular Structures by Light Scattering

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  February 26, 2019

  8:00 a.m. PST / 11:00 a.m. EST / 16:00 GMT / 17:00 CET

 

Overview

 

Comprehensive characterization of complex macromolecular structures is challenging since no single analytical technique provides adequate information regarding all the different distributions. Hyphenated techniques, that couple separation by asymmetric-flow field-flow fractionation (AF4) or size-exclusion chromatography (SEC) with a multi-detection system, do provide detailed insight into such structures. A versatile online multi-detection system includes multi-angle light-scattering (MALS), dynamic light scattering (DLS), ultraviolet (UV) and refractive-index (RI) detectors.

This webinar explores the application of SEC-MALS-DLS-UV-RI and AF4-MALS-DLS-UV-RI to understanding the molar mass, composition, size, distributions and structure of hybrid polymers and supramolecular structures. Examples include star copolymers, protein-poly(ethylene glycol) conjugates, large unilamellar vesicles, lipid droplets and exosomes.

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Key Learning Objectives

  • How size, size distributions and structures are determined by MALS and DLS combined with size-based separation
  • Capabilities and limitations of characterization by AF4/SEC-MALS-DLS-UV-RI
  • How AF4/SEC-MALS-DLS-UV-RI are applied to specific macromolecular and supramolecular characterization tasks

Who Should Attend

  • Scientists interested in detailed physico-chemical characterization of bionanoparticles, branched or hybrid polymers and related entities.
  • Lab managers seeking to explore new technologies for macromolecular and nanoparticle characterization.
 

Speaker

Ema Zagar,
Head, Dept. of Polymer Chemistry and Technology,
National Institute of Chemistry, Slovenia
 

Moderator

Britt Erikson
Senior Editor,
C&EN
 

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