CEN Webinars: Stronger Bonds
Measuring Size, Stability and Conformation of Biopolymers with Dynamic Light Scattering
Wednesday March 23rd 2016
USA 11:00am EST, 8:00 am PST, 16:00 GMT
SPEAKER
Speaker
John Champagne Ph.D.,
Senior Applications Scientist,
Northeast Regional Manager,
Wyatt Technology
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MODERATOR
Moderator
Britt Erickson,
Senior Editor,
C&EN
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OVERVIEW

Light scattering (LS), including classical and dynamic, has been widely employed to characterize protein solutions and other biomolecules. Classical or static light scattering, especially multi-angle light scattering (MALS), determines the absolute molecular weight of macromolecules in solution, facilitating the characterization of molecular heterogeneity and impurity profile. Dynamic light scattering (DLS), also known as quasi-elastic light scattering (QELS), directly measures the translational diffusion coefficient from which the hydrodynamic radius of a molecule is derived. Both static and dynamic light scattering can be used either in stand-alone (unfractionated, batch) mode or on-line coupled with separation techniques, such as size exclusion chromatography (SEC) or field-flow fractionation (FFF).

In this seminar, application examples will be presented for simultaneously measuring molecular size of proteins and biomolecules using the Plate Reader Plus. In addition, it will be discussed how to integrate DLS with MALS in a fractionation system and how the combined size and molar mass measurements can reveal unique insight into protein and biomolecule conformation and aggregation.

KEY LEARNING OBJECTIVES:

- Characterization of purified proteins and thermal stability over a range of solution conditions

- Measuring the kinetics of macromolecular assemblies

- Aggregate screening over several solution conditions, thermal conditions and time frames

- Measuring the size and stability of liposome and virus particles

- Crystallography screening of proteins over a range of solvent conditions

- Integration of DLS with MALS in a fractionation system and how the combined size and molar mass measurements can reveal unique insight into protein and biomolecule conformation and aggregation

WHO SHOULD ATTEND
  • • Researchers and lab technicians currently working with or planning on transitioning to DLS
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  • • Staff scientists interested in the number of unique roles that DLS can play in the modern analytical lab
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  • • Project Supervisors needing more information about cutting-edge technology for protein, biopolymer and nanoparticle characterization
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