(you guessed it, the Light Scattering Toolbox!)
11:00 am EDT, 8:00 am PDT, 16:00 BST
Antibody-Drug Conjugates (ADCs) present new challenges for biophysical characterization during R&D. A suite of analytical tools based on light scattering addresses many of these challenges, from determination of essential properties such as molar mass and drug-antibody ratio (DAR) to propensity for aggregation and identification of degradants. We present the Light Scattering Toolbox, based on multi-angle static light scattering (MALS) and dynamic light scattering (DLS) plus additional techniques, and discuss examples of its use in the service of ADC development.
- Which biophysical properties of ADCs are measured by different light scattering techniques, and the range of measurements
- How ADC formulations are rapidly screened for stability by high-throughput DLS
- How DAR is analyzed by triple-detection SEC- MALS
- How soluble and insoluble sub-micron aggregates are characterized by FFF-MALS