CEN Webinars: Stronger Bonds
Mass, Size, Composition:
Field Flow Fractionation of Biomolecules and Nanoparticles
plus Characterization by Light Scattering and ICP-MS
Tuesday October 22nd 2013
11:00 a.m. EDT, 10:00 a.m. CDT, 8:00 a.m. PDT, 15:00 GMT
Dr. Jeffrey Ahlgren,
Senior Applications Scientist,
Wyatt Technology Corporation
Susan Morrissey,
Assistant Managing Editor,
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Field Flow Fractionation coupled with Multi-Angle Light Scattering (MALS) and/or Dynamic Light Scattering (DLS) detection offers several advantages not found in other fractionation and characterization techniques such as size exclusion chromatography (SEC) including absolute, standards-free analysis and the absence of a stationary phase. FFF-MALS complements SEC-MALS when multiple means of characterization are required by regulatory agencies for product identification.

More recently, the combination FFF-MALS with ICP-MS has become important in environmental analysis and food characterization to evaluate the composition, size and number of particles present in solution. Recent technical advances include the development of hollow fiber cartridges which reduce the amount of sample required per run.


- How does FFF work to fractionate small and large particles in solution?

- What are the range and accuracy of MALS and DLS analyses applied to fractionated nanoparticles?

- What kinds of samples can be used with FFF and which detection types can be utilized with each sample type?

- How does FFF couple to ICP-MS and what advantages does it offer compared to other techniques?

  • • Researchers requiring accurate size distributions of nanoparticles not attained with batch dynamic light scattering, especially those who have encountered challenges with other fractionation techniques.
  • • Therapeutic manufacturers in need of orthogonal techniques to SEC-MALS or AUC for product characterization for regulatory review
  • • Laboratory managers or supervisors responsible for characterization of particles that are too large for traditional chromatographic approaches
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