USA 11:00am EST, 8:00 am PST, 16:00 GMT, 17:00 CET
Director of Analytical Services,
Wyatt Technology Corporation
Ultra-high performance size exclusion chromatography (UHP-SEC, a.k.a. SE-UHPLC) is gaining in popularity owing to the high speed and resolution of separation compared to standard HPLC-SEC. Online multi-angle light scattering (MALS) is the recognized method for obtaining absolute molecular weight and size, independently of column retention time, making it indispensable for accurate and reliable characterization by SEC. However, light scattering detectors designed for HPLC-SEC are in general not suitable for UHP-SEC. In this webinar we discuss the limitations of standard MALS detectors and present the capabilities of a MALS detector designed explicitly to meet the challenges of UHP-SEC. The utility of UHP-SEC combined with MALS - dubbed ŒµSEC-MALS¹ - as well as online dynamic light scattering will be demonstrated in the context of protein and synthetic polymer characterization.
- How µSEC-MALS overcomes the limitations of MALS instrumentation for UHP-SEC
- What new information can be gained by adding a µDAWN to the UHP SEC system
- Important tips and tricks in µSEC-MALS