Small, but Bright: µSEC-MALS Adds Light Scattering to UHP-SEC for Absolute Molecular Weight and Size
CEN Webinars: Stronger Bonds
Small, but Bright: µSEC-MALS Adds Light Scattering to UHP-SEC for Absolute Molecular Weight and Size
Tuesday November 10th 2015
USA 11:00am EST, 8:00 am PST, 16:00 GMT, 17:00 CET
SPEAKER
Speaker
Michelle Chen, Ph.D.,
Director of Analytical Services,
Wyatt Technology Corporation
Spacer
MODERATOR
Moderator
Jyllian Kemsley,
Senior Editor,
C&EN
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OVERVIEW

Ultra-high performance size exclusion chromatography (UHP-SEC, a.k.a. SE-UHPLC) is gaining in popularity owing to the high speed and resolution of separation compared to standard HPLC-SEC. Online multi-angle light scattering (MALS) is the recognized method for obtaining absolute molecular weight and size, independently of column retention time, making it indispensable for accurate and reliable characterization by SEC. However, light scattering detectors designed for HPLC-SEC are in general not suitable for UHP-SEC. In this webinar we discuss the limitations of standard MALS detectors and present the capabilities of a MALS detector designed explicitly to meet the challenges of UHP-SEC. The utility of UHP-SEC combined with MALS - dubbed ŒµSEC-MALS¹ - as well as online dynamic light scattering will be demonstrated in the context of protein and synthetic polymer characterization.

KEY LEARNING OBJECTIVES:

- How µSEC-MALS overcomes the limitations of MALS instrumentation for UHP-SEC

- What new information can be gained by adding a µDAWN to the UHP SEC system

- Important tips and tricks in µSEC-MALS

WHO SHOULD ATTEND
  • • Scientists, and lab technicians currently working with, or planning on transitioning to, UHP-SEC
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  • • Lab managers looking for advanced characterization capabilities for proteins and polymers
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