New Peptide Dissociation Method For Mass Spec | January 19, 2009 Issue - Vol. 87 Issue 3 | Chemical & Engineering News
 
 
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Volume 87 Issue 3 | p. 57 | Concentrates
Issue Date: January 19, 2009

New Peptide Dissociation Method For Mass Spec

Femtosecond laser-induced ionization/dissociation is a novel technique for analyzing finicky peptides in proteomics experiments.
Department: Science & Technology

Analysis of peptides in proteomics experiments requires the use of dissociation methods that break the peptides into smaller pieces to determine the amino acid sequence. Gavin E. Reid and coworkers of Michigan State University describe a new dissociation method, called femtosecond laser-induced ionization/dissociation (fs-LID), that overcomes some of the limitations of other dissociation methods (J. Am. Chem. Soc., DOI: 10.1021/ja8089119). In fs-LID, ultrashort laser pulses (<35 femtosecond) deposit sufficient . . .

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