Nixing Nitric Oxide | April 1, 2013 Issue - Vol. 91 Issue 13 | Chemical & Engineering News
 
 
1
Facebook
Volume 91 Issue 13 | p. 12 | News of The Week
Issue Date: April 1, 2013 | Web Date: March 29, 2013

Nixing Nitric Oxide

Bioinorganic Chemistry: Reductase enzyme mimic provides insight on how pathogenic bacteria thwart NO attack
Department: Science & Technology
News Channels: Materials SCENE, Biological SCENE, JACS In C&EN
Keywords: nitric oxide, nitrous oxide, reductase
[+]Enlarge
This diiron complex efficiently mediates reduction of NO. (H atoms not shown.)
Credit: Deidra Gerlach
09113-notw6-fenobcxd
 
This diiron complex efficiently mediates reduction of NO. (H atoms not shown.)
Credit: Deidra Gerlach

Nitric oxide is a double-edged sword. It’s a key signaling molecule that controls blood pressure and nerve impulses, yet when concentrations of the simple gas molecule are too high, it’s toxic to cells. But this toxicity can be a valuable asset as well because it allows NO to serve as a central immune defense mechanism to destroy pathogenic bacteria such as Helicobacter pylori that invade the digestive system.

Some bacteria have countered by evolving to produce iron-based reductase enzymes that repel NO by reducing it to harmless nitrous oxide, N2O. Understanding how such enzymes work could lead to new drugs to thwart the bacterial NO defense mechanism and prevent infections.

A team of chemists at the University of Michigan has now taken a step toward this goal by developing a diiron complex that serves as the first functional model for the catalytic site of an NO reductase enzyme (J. Am. Chem. Soc., DOI: 10.1021/ja309782m).

Nicolai Lehnert and coworkers determined how the diiron complex bearing a nonheme ligand system with an additional flavin cofactor binds two molecules of NO and, in a two-electron reduction process, converts them to N2O and water. The Michigan system is significantly faster and more efficient in generating N2O than other reductase models.

Besides new drugs, the mechanistic insight from studying the diiron complex could be important in nonbiological applications, Lehnert notes, such as the use of electrocatalysts to decompose nitrogen oxide pollutants in automobile and power plant exhaust.

“There is a growing interest in flavodiiron NO reductases owing to their possible roles in the detoxification of NO,” notes Peter C. Ford, whose group at the University of California, Santa Barbara, studies NO bioactivity. “Lehnert and coworkers have provided a very interesting diiron functional model,” he says, “that certainly adds to the growing recognition of the importance of nonheme iron complexes in the chemical biology of NO.”

 
Chemical & Engineering News
ISSN 0009-2347
Copyright © American Chemical Society
Comments
Barry E  (April 1, 2013 10:11 AM)
Are there any studies that show NO is unsafe to use as a dietary supplement in conjunction with weight training?
TOM BAAR  (July 7, 2013 11:53 PM)
WHAT ARE THE NEGATIVES ABOUT NO TO RAISE YOUR NITRIC OXIDE LEVELS AND THE TEST STRIPS USED TO SHOW YOUR LEVELSWITH NO SUPPLEMENTS ALSO ANY RECOMENDED COS.
Leave A Comment