Issue Date: September 10, 2007
Tracking Down Acetyltransferases
By employing a functional group never before used in protein labeling, a new chemical probe could enable closer scrutiny of an enormous and diverse enzyme family (Angew. Chem. Int. Ed., DOI: 10.1002/anie.200702485). A team led by Philip A. Cole at Johns Hopkins School of Medicine and Neil L. Kelleher of the University of Illinois, Urbana-Champaign, designed a probe for capturing acetyltransferases, which are enzymes that use the cofactor acetyl coenzyme A (CoA) to acylate their substrates. At the probe's heart is a thiocarbamate sulfoxide (shown, left), an electron-poor functional group that's attacked by the thiol group of acetyltransferases' nucleophilic cysteine residues (Nu). The reaction releases the CoA directing group and tags the enzyme with a biotin analog for detection and purification (right). The team anticipates that the probe will be useful in identifying unknown CoA-binding proteins involved in signaling or gene regulation and that thiocarbamate sulfoxides will be broadly applicable in protein labeling.
- Chemical & Engineering News
- ISSN 0009-2347
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