Lanthanides Expand Spectroscopic Arsenal | Chemical & Engineering News
Latest News
Web Date: May 16, 2007

Lanthanides Expand Spectroscopic Arsenal

Metal ions' luminescent and magnetic properties enhance protein detection and structural studies
Department: Science & Technology
News Channels: JACS In C&EN
[+]Enlarge
OVERVIEW
Crystal structure of a lanthanide binding tag reveals the position of Tb3+ ions (blue spheres) within the peptide sequence.
Credit: Courtesy of Karen N. Allen
8521scicimg1b
 
OVERVIEW
Crystal structure of a lanthanide binding tag reveals the position of Tb3+ ions (blue spheres) within the peptide sequence.
Credit: Courtesy of Karen N. Allen

Lanthanide metal ions are gaining in popularity as a means to increase the power of spectroscopy for studying proteins. In the latest example, Karen N. Allen of Boston University, Barbara Imperiali of MIT, and Harald Schwalbe of Johann Wolfgang Goethe University, in Frankfurt, Germany, teamed up to create peptide-based tags that bind two lanthanide ions and can be added easily to proteins (J. Am. Chem. Soc., DOI: 10.1021/ja070480v).

Attached to the protein ubiquitin and loaded with Tb3+, the tags provided strong luminescence signals for protein detection. When loaded with the paramagnetic lanthanides Tm3+ and Eu3+, the tags helped to orient ubiquitin proteins for NMR spectroscopy structure determination.

In a second paper, the researchers used tags with Tb3+ to determine the phases of diffracted X-rays to solve ubiquitin's crystal structure (J. Am. Chem. Soc., DOI: 10.1021/ja070481n).

The tags could become valuable tools, the researchers say, especially for studying eukaryotic proteins for which existing approaches, such as the incorporation of selenium for X-ray phasing, have proven difficult.

 
Chemical & Engineering News
ISSN 0009-2347
Copyright © American Chemical Society

Leave A Comment

*Required to comment