Issue Date: June 25, 2012
Single Amino Acid Forms Fibrils
The amino acid phenylalanine assembles into toxic fibrils, researchers at Tel Aviv University and the University of Zurich report (Nat. Chem. Biol., DOI: 10.1039/nchembio.1002). This is the first time that individual amino acid molecules have been shown to assemble into fibrils.
The findings suggest that the disease phenylketonuria, which is characterized by phenylalanine accumulation and may lead to severe problems with brain development, could be a type of amyloid disease, which is caused by toxic protein fibrils. The work could lead to possible treatments for phenylketonuria.
Ehud Gazit, Lihi Adler-Abramovich, and coworkers observed millimolar concentrations of phenylalanine self-assemble into fibrillar structures. The fibrils have a positive response to the dyes Congo red and thioflavin T, which are standard tests for amyloids. The fibrils also elicit cytotoxic and immunological responses in rodents and rabbits.
The structures differ slightly from other amyloids, however. In conventional amyloids, hydrogen bonds that hold fibrils together are oriented parallel to the fibril axis. In the phenylalanine fibrils, hydrogen bonds are instead perpendicular to the axis.
The lack of amide bonds in the single-molecule-based fibrils likely accounts for the different orientation, Gazit says. Unlike in peptide-based fibrils, “the interactions here apparently stem mainly from side-chain interactions that lead to alternative packing,” he adds.
The researchers found the single-molecule-based phenylalanine fibrils in postmortem brain tissue from individuals with phenylketonuria. “The implications are significant,” Gazit says. If phenylketonuria is indeed confirmed to be an amyloid disease, it “may be treated not only by a very severe diet but also by the use of antiaggregation compounds that are being developed for other indications.”
“The finding that phenylalanine assembles into toxic fibers is striking,” says David S. Eisenberg, an amyloid expert at UCLA. The new study “gives strong evidence that phenylketonuria is an aggregation disease. The mechanism of toxicity of phenylalanine fibers could be quite different than in other diseases, though, because it appears that these fibers may have a different structure than classical amyloid.”
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