ADVERTISEMENT
2 /3 FREE ARTICLES LEFT THIS MONTH Remaining
Chemistry matters. Join us to get the news you need.

If you have an ACS member number, please enter it here so we can link this account to your membership. (optional)

ACS values your privacy. By submitting your information, you are gaining access to C&EN and subscribing to our weekly newsletter. We use the information you provide to make your reading experience better, and we will never sell your data to third party members.

ENJOY UNLIMITED ACCES TO C&EN

Analytical Chemistry

Putting The Squeeze On Cells

Microfluidic device allows scientists to examine cell size and stiffness simultaneously

by Journal News and Community
July 9, 2012 | APPEARED IN VOLUME 90, ISSUE 28

A new microfluidic device can rapidly analyze the size and rigidity of cells all at once (Anal. Chem., DOI: 10.1021/ac300264v). Cells are a bit like pillows, ranging from hard to soft. Previous high-throughput methods to measure cell stiffness infer the property from the time it takes a cell to travel through a microfluidic channel: Firm cells take longer to squeeze through tiny passageways than soft cells do. But MIT’s Klavs F. Jensen wanted to build a device that also takes into account cell size to improve stiffness measurements. His team’s device consists of a microfluidic path running between two electrodes. By measuring electrical resistance, the researchers can determine the size of a cell passing through. As cell size increases, so does resistance. They can also measure the cell’s transit time via how long this resistance spike lasts. The researchers tested the device on HeLa cells, some of which they softened with a toxin that breaks down the cytoskeleton. As predicted, softer cells took less travel time than harder, untreated cells, and smaller cells traveled faster than larger cells.

X

Article:

This article has been sent to the following recipient:

Leave A Comment

*Required to comment