Volume 91 Issue 28 | p. 25 | Concentrates
Issue Date: July 15, 2013

CRAPome Aggregates Contaminant Info For Protein Interactions

Database helps researchers identify nonspecific binding partners in affinity purification MS experiments
Department: Science & Technology
News Channels: Analytical SCENE, Biological SCENE
Keywords: mass spectrometry, protein interactions

Affinity purification combined with mass spectrometry is a popular way to determine protein interaction partners. The problem is that the bait proteins used to find interacting proteins can also snag nonspecific partners. Scientists had not found a good way to benefit from the experience of others who have previously caught such contaminants, but a new online database assembled by a large international team of researchers could help. The cleverly named contaminant repository for affinity purification—that’s . . .

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Chemical & Engineering News
ISSN 0009-2347
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