Cheerios-Fed Fungi Yield Biofilm Blockers | Chemical & Engineering News
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Web Date: October 16, 2014

Cheerios-Fed Fungi Yield Biofilm Blockers

Natural Products: Soil fungi dine on breakfast cereal, then construct metabolites that block yeast biofilms
Department: Science & Technology | Collection: Life Sciences
News Channels: Biological SCENE, Organic SCENE
Keywords: mycology, fungi, bionectriol, biofilm, Candida albicans, Cheerios, polyketide glycoside
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Eat Your Os
In a lab, fungi flourish on Cheerios, allowing the organisms to synthesize consistent mixtures of metabolites for study by natural products chemists. The cereal pieces are about 12 mm wide.
Credit: Jianlan You
Photograph of a Cheerio piece engulfed by fungus.
 
Eat Your Os
In a lab, fungi flourish on Cheerios, allowing the organisms to synthesize consistent mixtures of metabolites for study by natural products chemists. The cereal pieces are about 12 mm wide.
Credit: Jianlan You

Convincing fungi to trot out their whole gamut of metabolites is a persistent challenge of natural product chemists. These researchers hunt for novel compounds with possible therapeutic value within the chemical soup that the fungi produce. Now, one team has hit upon a curiously effective and consistent way to prod the organisms to start synthesizing: Cheerios inside bags. Scientists grew a soil fungus for four weeks in a bag full of Cheerios and discovered a new compound that can block biofilm formation by an infectious yeast (J. Nat. Prod. 2014, DOI: 10.1021/np500531j).

A yeast infection brought on by Candida albicans will vex, at one time or another, about 75% of healthy women. In immunocompromised patients, the ubiquitous fungus can be intractable. Once it gets a foothold, C. albicans often puts up a biofilm, a shield of polymeric slime that repels immune system attacks and antifungal drugs. A drug that could block biofilm formation would prevent recalcitrant infections and make antifungal treatments more effective, says Robert H. Cichewicz, a natural products chemist at the University of Oklahoma.

His lab has been searching through fungi metabolites for novel compounds with antibiofilm properties. But cultivating fungi using standard laboratory protocols—Erlenmeyer flasks filled with broth—often leads to the same common mycotoxins.

Cichewicz was convinced the fungi could make different, novel compounds if he could find the proper growing conditions. So in 2007, Cichewicz started hunting Walmart aisles and his kitchen cabinets for alternative fungus fodder. The chemist hit the jackpot with a box of stale Cheerios.

“Pretty soon we were getting rich, luxuriant lawns on top of the Cheerios,” Cichewicz says. The fungi grew in all the crevices, leaving behind “fuzzy doughnut shells where the Cheerio used to be.” The lawns were colorful, indicating large numbers of fungal metabolites. Also, Cheerios overcame a common problem in growing fungi. Standard growth media varies in composition from batch to batch. These small variations can alter fungi growth, meaning researchers can’t consistently produce the same set of metabolites with each experiment. However, one Cheerio is the same as another, box to box, batch to batch, today or years from now.

The idea to put the Cheerios in a bag instead of a flask came in 2011, when Cichewicz stumbled upon a how-to blog for growing psychedelic mushrooms. Those growers use big, breathable plastic bags called mushroom bags. One 50-cent bag, Cichewicz calculated, would provide the same growing surface area as 18 Erlenmeyer flasks. That would cut back on graduate student hours spent washing glassware, he thought. When his team took one promising fungus, Bionectria ochroleuca, and compared its growth on Cheerios in flasks versus in mushroom bags, they found that the species produced a similar metabolite profile under both conditions.

The fungus made three new bioactive compounds the scientists call bionectriols. The compounds were potent in preventing both growth and biofilm formation of C. albicans. Also, the bionectriols enhanced the potency of amphotericin B, one of physicians’ last lines of defense for treating fungal infections.

Nicholas H. Oberlies of the University of North Carolina, Greensboro, agrees that the antibiofilm properties of the bionectriols are intriguing. But he also is impressed with Cichewicz’s Cheerios-based cultivation method. “The clever thing is that he got General Mills to take the variability out for him,” Oberlies says.

Cichewicz continues to extol the benefits of Cheerios for growing fungi. “There is no other cereal that even comes close,” he says. “And believe me, I’ve tested the whole cereal aisle now.”

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This new polyketide glycoside was made by a soil fungus from Oklahoma (Bionectria ochroleuca) and shows potent antibiofilm activity.
Credit: J. Nat. Prod
Structure of bionectriol C.
 
This new polyketide glycoside was made by a soil fungus from Oklahoma (Bionectria ochroleuca) and shows potent antibiofilm activity.
Credit: J. Nat. Prod
 
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Comments
Alycia (October 20, 2014 2:04 PM)
Will there be a new anti fungal product for the feet and hands coming to the market soon?
rebecca (May 21, 2015 1:05 PM)
Cichewicz says he tested the whole cereal aisle. How could it be that Cheerios, with only 1g of sugar per serving and with oats as the first ingredient be the cereal that is best for the experiment? This is distressing to me because I have candida overgrowth and have been wanting to eat Cheerios. Right now I can't get to a grocery store and the safest cereal I can eat from my nearby convenience store is Cheerios. I know that Cheerios also contain wheat starch, but other cereals are much worse. I would think that Froot Loops, which have sugar as their first ingredient, would produce more fungi for this experiment. What makes Cheerios better for growing fungi?
I would think that almost every other cereal would grow more fungi as most have more sugar and or more wheat. Does any one have an idea why Cheerios would cause more fungi to grow?

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