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Web Date: February 2, 2018

How lanthanides keep volcanic bacteria alive

Coordination complex reveals why rare-earth elements give methanol dehydrogenase enzyme a boost
Department: Science & Technology
News Channels: Biological SCENE, Organic SCENE, JACS In C&EN
Keywords: Biocatalysis, lanthanide, enzyme, methanol dehydrogenase, Methylacidiphilum fumariolicum, volcanic bacteria
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Methylacidiphilum fumariolicum uses a methanol dehydrogenase enzyme that contains a lanthanide ion (cyan) bound to a pyrroloquinoline quinone cofactor (oxygen shown in red, nitrogen in blue).
Credit: A. McSkimming
Illustration of active site of methanol dehydrogenase enzyme with its cofactor bound to a lanthanide ion.
 
Methylacidiphilum fumariolicum uses a methanol dehydrogenase enzyme that contains a lanthanide ion (cyan) bound to a pyrroloquinoline quinone cofactor (oxygen shown in red, nitrogen in blue).
Credit: A. McSkimming

Ten years ago, in a steaming volcanic mudpot in Italy, microbiologists discovered a bizarre bacterium—the first known organism that couldn’t live without lanthanides. To help it feed on methane, Methylacidiphilum fumariolicum relies on a methanol dehydrogenase (MDH) enzyme that has a rare-earth element such as cerium or lanthanum at its heart.

Now, Eric J. Schelter of the University of Pennsylvania has made a complex that mimics the enzyme’s active site, shedding light on the lanthanide’s crucial role in the microbe’s metabolism (J. Am. Chem. Soc. 2017, DOI: 10.1021/jacs.7b12318).

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This quinoline quinone ligand, serving as a stand-in for the enzyme’s cofactor, coordinates to a lanthanum ion to create a catalytically active complex.
Credit: J. Am. Chem. Soc.
Structure of a quinoline quinone that coordinates to a lanthanum ion.
 
This quinoline quinone ligand, serving as a stand-in for the enzyme’s cofactor, coordinates to a lanthanum ion to create a catalytically active complex.
Credit: J. Am. Chem. Soc.

Lanthanide ions have very poor bioavailability, because they tend to precipitate out of neutral water. But in the hot, acidic conditions of the Solfatara volcanic crater on the outskirts of Naples, hardy microbes have access to a rare-earth smorgasbord at concentrations of 2–3 µM. Indeed, M. fumariolicum depends on a supply of lanthanides to thrive (Environ. Microbiol. 2013, DOI: 10.1111/1462-2920.12249). “I remember when I first heard about this, and I was just stunned,” says Rachel N. Austin of Barnard College, a metalloprotein expert who was not involved in the research.

MDH converts methanol into formaldehyde, a key step in methane metabolism. The enzyme contains a pyrroloquinoline quinone (PQQ) cofactor, which is normally bound to a calcium ion. In M. fumariolicum, though, X-ray crystallography has shown that a lanthanide ion takes calcium’s place.

To understand exactly how the lanthanide-MDH works, Schelter’s team developed a model compound that was much easier to manipulate than the enzyme itself. PQQ can bind to metal ions in many different ways, so the researchers created a more obedient stand-in without pyrrole or carboxylic acid groups, and with bulky cyclohexyl groups, to ensure it coordinated with a lanthanum ion in the right way. The resulting complex converted a benzyl alcohol—a convenient substitute for methanol—into its aldehyde and did so catalytically with the help of additional oxidant and base.

Density functional theory calculations based on the model complex helped the team to figure out the reaction mechanism, which involved a hydride transfer step. Schelter says that the lanthanum ion lowers the activation barriers to key intermediates in the mechanism, because it is better at accepting electron pairs from the ligand than calcium. “The bug is getting something better out of using the rare earths,” he says.

Austin says that model studies like this can help to address a fundamental question in biology: Do enzymes simply adopt the most available metal ions, or is nature more selective than that? Schelter’s model suggests that using a lanthanide in MDH, rather than calcium, could have given the mudpot bacteria an evolutionary advantage.

Meanwhile, M. fumariolicum is no longer unique. Last year, studies on water samples from the Deepwater Horizon oil spill in 2010 suggested that microbes feasting on methane released during the disaster had been using lanthanide-MDH (Sci. Rep. 2017, DOI: 10.1038/s41598-017-11060-z). Even leaf-dwelling bacteria found on the campus of San Jose State University apparently use lanthanum with their MDH (Science 2015, DOI: 10.1126/science.aaa9091). “The microbiologists are finding these things everywhere,” Schelter says. “I think we’re just at the beginning of this story.”


This article has been translated into Spanish by Divulgame.org and can be found here.

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Arjan Pol of Radboud University takes a sample from a mud pot at the Solfatara volcanic crater in Italy, where in 2007 he and his colleagues found bacteria that consume methane.
Credit: Paola Mariani
Photo of a researcher crouching on the edge of a bubbling pool of mud next to a plastic bottle and taking a sample with a scoop on the end of a long handle.
 
Arjan Pol of Radboud University takes a sample from a mud pot at the Solfatara volcanic crater in Italy, where in 2007 he and his colleagues found bacteria that consume methane.
Credit: Paola Mariani
 
Chemical & Engineering News
ISSN 0009-2347
Copyright © American Chemical Society
Comments
Dalil Rahman (February 7, 2018 4:02 PM)
Very Interesting
Shankar (February 8, 2018 1:15 PM)
I am just curious if other Lanthanides have been probed? Specially, Gd or Lu?
Dr David Thomas (February 15, 2018 4:37 AM)
"An enzyme used to metabolize methane binds to lanthanide ions, which may have given the microbe an evolutionary advantage". I copied and pasted that from your email.
But it doesn't, does it. According to the article it converts methanol to formaldehyde.
It's like being invited to dinner and given tea and biscuits!
Steve Ritter (February 24, 2018 7:29 AM)
More wordplay from UPenn's chemical poet Andrew Roxburgh McGhie

Lanthanides for the Living

M. fumariolicum
Is a bizarre bacterium
It needs a methanol dehydrogenase enzyme
That has cerium or lanthanum deep in its spine
And for this bacterium to stay alive
It needs hot, acidic conditions for it to thrive
There is one place for which this enzyme, it caters
It is the Solfatara volcano’s crater
The enzyme contains a pqq co-factor
In which pyrroloquinolinequinone is the actor
Now Eric Schelter, a fine chemist at Penn
Has gone and done it, at least, once again
He designed a new quinolinequinone ligand
Which mimics the catalytic activity at hand
Converting benzyl alcohol to aldehyde in place
Without needing additional oxidant or base
And Density Matrix Theory on the model complex
Solved the reaction mechanism in this context
This was found to involve a hydride transfer step
At which this Lanthanide complex is very adept
So this La-MDH enzyme, based on work of Schelter
Is mud-pot bacteria’s evolutionary helper
Now this M fumariolicum is no longer unique
It has also been found working in waters deep
For in the Deepwater Horizon’s oil samples
La-MDH-based microbes were found, for example
And so in this story, which is just beginning
It appears that our microbiologists are winning.

Andrew Roxburgh McGhie 02.24.2018

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