Thoroughly Analyzing Antibody Responses | November 10, 2008 Issue - Vol. 86 Issue 45 | Chemical & Engineering News
Volume 86 Issue 45 | p. 36 | Concentrates
Issue Date: November 10, 2008

Thoroughly Analyzing Antibody Responses

A new microarray-based assay singles out and comprehensively characterizes individual antibody-producing immune cells
Department: Science & Technology
A polymer slab contains an array of microscopic wells for holding individual immune cells.
Credit: Carmen Drahl/C&EN
8645scon_slabcxd1_opt
 
A polymer slab contains an array of microscopic wells for holding individual immune cells.
Credit: Carmen Drahl/C&EN

By adapting tools for printing protein microarrays, researchers have developed an assay that singles out and comprehensively characterizes antibody-producing cells that contribute to an immune response (Proc. Natl. Acad. Sci. USA, DOI: 10.1073/pnas.0805470105). The approach could help advance clinical research on infectious and autoimmune diseases and facilitate the design of vaccines. Established immunoassays either obscure the contributions of individual immune system cells in the overall immunological response or are not capable of screening many cell and antibody characteristics at once. A multi-institution team led by Massachusetts Institute of Technology chemical engineer J. Christopher Love has designed an assay that can do both. In the technique, individual immune cells are first segregated into an array of subnanoliter-sized wells. A glass slide placed in contact with the wells then picks up antibodies secreted by each cell. Analysis of the resulting array provides detailed quantitative profiles of the antibodies, which can be correlated to the individual immune cells. The team successfully used the assay to track antibody responses of mice after they received an immunization.

 
Chemical & Engineering News
ISSN 0009-2347
Copyright © American Chemical Society

Leave A Comment

*Required to comment