Counting CD4+ T Cells By Chemiluminescence | December 7, 2009 Issue - Vol. 87 Issue 49 | Chemical & Engineering News
Volume 87 Issue 49 | p. 41 | Concentrates
Issue Date: December 7, 2009

Counting CD4+ T Cells By Chemiluminescence

A potential low-cost microfluidic device could help HIV/AIDS patients monitor their immune systems
Department: Science & Technology
Keywords: microfluidics, diagnostic devices, AIDS, HIV, CD4
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A light-free microfluidic device traps CD4+ T cells and detects them via chemiluminescence.
Credit: Courtesy of Samuel Sia
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A light-free microfluidic device traps CD4+ T cells and detects them via chemiluminescence.
Credit: Courtesy of Samuel Sia

A low-cost, portable device for measuring the number of CD4+ T-lymphocyte cells in blood could significantly improve the management of HIV/AIDS in patients. CD4 is a receptor on the surface of T cells in the immune system, and a CD4+ T-cell count below 200 cells per μL signals the onset of AIDS. Columbia University’s Samuel K. Sia and coworkers are now reporting a microfluidic device that uses chemiluminescence detection to track CD4+ T cells at the clinically significant 200 cells per μL level (Anal. Chem., DOI: 10.1021/ac902144w). “Chemiluminescence detection works without the need of an external light source, which takes us a step closer to the development of a portable diagnostics device with minimal instrumentation,” Sia says. The researchers isolate CD4+ T cells from whole blood by trapping the cells on microfabricated pillars that are coated with anti-CD4 antibody. To avoid measuring monocytes, which also express CD4, they detect the T cells with anti-CD3 antibodies that are conjugated to horseradish peroxidase. The researchers incubate the cells with the chemiluminescent substrates luminol and hydrogen peroxide and then measure the photocurrent produced as a result of the chemiluminescent reaction.

 
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