Volume 90 Issue 50 | p. 9 | News of The Week
Issue Date: December 10, 2012

A New Look At Harvesting Light

Biochemistry: Molecules key to color vision can be manipulated to alter their light-absorbing properties
Department: Science & Technology | Collection: Life Sciences
News Channels: Biological SCENE, Analytical SCENE
Keywords: vision, retinal, chromophore, protein engineering
Credit: Babak Borhan
Engineered protein binding retinal within its binding pocket.
Credit: Babak Borhan
Credit: Babak Borhan
Cuvettes containing the same concentration of retinal but different proteins.
Credit: Babak Borhan

Retinal (red) bound to engineered proteins absorbs different colors, depending on the protein sequence. Cuvettes contain the same concentration of retinal but different proteins.

A better understanding of the molecules responsible for color vision—as well as a host of practical applications—may result from new chemical research on these light-absorbing chromophores.

Chemists at Michigan State University report that the protein environment can be manipulated to change a chromophore’s light absorption properties (Science, DOI: 10.1126/science.1226135). Ultimately, they say, the work could lead to new biobased sensors, light-harvesting proteins for solar energy, and new protein tags for biological research.

The pigmented rhodopsins, the light receptor proteins in color vision, all use the same molecule—11-cis-retinal—to absorb light across the visible spectrum. Nature has found a way to regulate 11-cis-retinal’s wavelength of maximum absorption in the human eye over a very large range, from about 420 to 560 nm, to detect various colors. Babak Borhan, James H. Geiger, and colleagues wanted to know what factors in the protein make such regulation possible. And they wanted to “red shift” the absorption profile as far as possible (toward longer wavelengths) to investigate the feasibility of expanding the visual palette.

To achieve their goals, they used cellular retinol-binding protein (CRBP) to mimic the electronic properties of rhodopsin-retinal complexes. They engineered CRBP so that it binds all-trans-retinal—a stable form of retinal that is easier to study than 11-cis-retinal—instead of retinol.

Engineering CRBP required two key mutations. First, they inserted a lysine in the right place to form an iminium bond with the retinal. “After we made that first mutation, that lysine was close to another lysine,” Borhan says, making the inserted one unreactive, so they replaced the second lysine with leucine. They then manipulated the electrostatic environment around the chromophore with other mutations. The resulting engineered proteins have absorption maxima (λmax) that range from 425 to 644 nm.

The researchers were surprised that their modifications pushed the absorption profile to wavelengths beyond values computational models had predicted to be possible. The most red-shifted protein, which has nine mutations, has a λmax of 644 nm, whereas previously no one thought retinal could have a λmax longer than about 620 nm, Borhan says.

“I think we have the opportunity to go even farther than 644 nm,” he says. “I’m not sure how much more we can go, but I don’t think we’re at the upper limit yet.”

In addition to having thus achieved a better understanding of the factors that lead to color vision, the Michigan State researchers plan to use the protein complexes as genetically encoded tags to label other proteins. “We have the ability to dial in whatever color we want, depending on the protein sequence,” Borhan says. They are also developing variants that bind fluorescent molecules. “Fluorescence is really the name of the game for protein fusion tags,” he says.

“We believe that our work provides a ‘toolbox’ of principles and strategies that can be used for controlling the spectroscopic properties of not just retinal, but potentially a variety of chromophores,” Geiger says.

The study “is a stunning example of design principles,” says Samie R. Jaffrey, a biologist at Weill Cornell Medical College. Borhan, Geiger, and coworkers have “figured out design principles for how you can rationally control the environment around a chromophore to change its absorbance properties.” He remains unsure, however, that the proteins will be practical protein tags.

Chemical & Engineering News
ISSN 0009-2347
Copyright © American Chemical Society

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