If you have an ACS member number, please enter it here so we can link this account to your membership. (optional)

ACS values your privacy. By submitting your information, you are gaining access to C&EN and subscribing to our weekly newsletter. We use the information you provide to make your reading experience better, and we will never sell your data to third party members.


Analytical Chemistry

Bacterial Fight Club Produces New Metabolites

Bacteria cocultures produce metabolites that aren’t seen in cultures of individual bacterial species

by Celia Henry Arnaud
June 15, 2015 | A version of this story appeared in Volume 93, Issue 24

When bacteria are forced to face off against each other, the fight can induce them to produce metabolites they don’t make when they’re hanging out by themselves. To study this phenomenon, Brian O. Bachmann, John A. McLean, and coworkers at Vanderbilt University pit bacterial strains against each other two at a time in cocultures to find new polyketide natural products for potential drugs (ACS Chem. Biol. 2015, DOI: 10.1021/acschembio.5b00001). The researchers analyze the coculture extracts with liquid chromatography-ion mobility mass spectrometry. Then they process the data with an algorithm that organizes spectral features according to the way they vary across coculture conditions to create a visually interpretable heat map. By subtracting the monoculture feature maps from the coculture feature maps, the researchers identify metabolites that are produced only in coculture. When the Vanderbilt team cocultured a Nocardiopsis strain with other kinds of bacteria, almost 14% of the detected metabolites were produced only in cocultures. In addition, the bacteria produced different metabolites in response to different sparring partners. One metabolite from a coculture of Nocardiopsis ΔApoS and Rhodococcus wratislaviensis—dubbed ciromicin A—contains an unusual pyrrolidinol substructure and is toxic to cancer cells.

Heat maps of metabolites produced in mono- and cocultures of bacteria. Subtracting monocultures from coculture reveals metabolites produced only in coculture.
Credit: ACS Chem. Biol.
Heat maps of metabolites generated in bacterial cocultures and monocultures are used to identify previously unknown natural products.


This article has been sent to the following recipient:

Chemistry matters. Join us to get the news you need.