Improved route to single-base genome editing | April 25, 2016 Issue - Vol. 94 Issue 17 | Chemical & Engineering News
Volume 94 Issue 17 | p. 5 | News of The Week
Issue Date: April 25, 2016 | Web Date: April 21, 2016

Improved route to single-base genome editing

Modified CRISPR-Cas9 technique corrects disease point mutations by efficiently changing single bases in DNA
Department: Science & Technology
News Channels: Biological SCENE
Keywords: genomics, base editor, point mutation, DNA, Cas9, CRISPR-Cas9
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Targeted
A base editor, consisting of a modified Cas9, cytidine deaminase, and uracil glycosylase inhibitor (not shown), converts C to U in a targeted C:G base pair. DNA repair and DNA replication convert the resulting U:G mismatch to T:A (shown) or U:A.
Credit: Adapted from Nature
Scheme shows how base editing technique first converts C to U in targeted G:C base pair and how DNA repair or DNA replication then converts the resulting G:U mismatch to A:U or A:T.
 
Targeted
A base editor, consisting of a modified Cas9, cytidine deaminase, and uracil glycosylase inhibitor (not shown), converts C to U in a targeted C:G base pair. DNA repair and DNA replication convert the resulting U:G mismatch to T:A (shown) or U:A.
Credit: Adapted from Nature

In a new approach to genome editing called “base editing,” researchers have engineered the CRISPR-Cas9 enzyme to modify individual DNA bases more efficiently and more accurately than with existing gene-editing methods.

Many genetic diseases arise from single-base mutations, or point mutations. David R. Liu, postdoc Alexis C. Komor, and coworkers at Harvard University developed the new base-editing process and showed that it can correct point mutations in living cells, suggesting the method could lead to therapies that correct genetic errors that cause human diseases (Nature 2016, DOI: 10.1038/nature17946).

Researchers currently use zinc finger nucleases, transcription-activator-like effector nucleases, and CRISPR-Cas9 for genome editing. All three tools initiate genome editing by cutting both strands of DNA. Cellular machinery reduces the efficiency of these editors’ ability to correct point mutations by trying to repair such double-strand breaks with so-called indels, random DNA insertions or deletions.

By avoiding double-strand breaks, the new approach causes far fewer indels and corrects point mutations more efficiently. Liu and coworkers began by using a Cas9 mutant (dCas9) that doesn’t cut DNA but retains native Cas9’s ability to bind to target DNA sequences. In this case, the team wanted to target C:G base pairs and change them to T:A. They made their first base editor by fusing the mutant to cytidine deaminase, which converts cytosine (C) to the RNA base uracil (U), thus changing targeted C:G base pairs to U:G. Cellular DNA repair and replication machinery can correct such U:G mismatches to U:A or T:A.

But cells’ DNA repair machinery can also change U:G back to C:G, so the researchers made a second base editor by appending to their first base editor a small protein called uracil glycosylase inhibitor, which prevents that switchback. In human cells, the second base editor increased the efficiency of base editing several-fold, compared with the first. Liu and coworkers then restored one of the DNA-cutting active sites in dCas9, creating a third base editor that further increases efficiency by favoring cellular repair of U:G to U:A or T:A over the switch back to C:G.

In tests in cell culture, Liu and coworkers observed that conventional CRISPR-Cas9 corrected 14 point mutations about 1% of the time, while causing about 5% indels. Their second base editor corrected about 10% of the same mutations and caused almost no indels, whereas the third was about 30% efficient and caused about 1% indels. Both the second and third base editors are useful, Liu notes, depending on whether mutation efficiency or avoiding indels is the more important goal of a particular experiment.

The researchers demonstrated the power of the technique by using it to reverse point mutations associated with Alzheimer’s disease and cancer in mouse and human cell lines, with minimal indel formation.

Emmanuelle Charpentier of the Max Planck Institute for Infection Biology, who helped discover the CRISPR-Cas9 technique, comments that “the study shows once again the power and versatility of the CRISPR-Cas9 mechanism.” The work “predicts future exciting applications in tailored genome and epigenome base editing.”

This article has been translated into Spanish by Divulgame.org and can be found here.

 
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