Issue Date: January 9, 2017
Biocatalytic cofactor recycled efficiently
Oxidoreductase enzymes are used extensively as biocatalysts for industrial and academic reactions, including pharmaceutical synthesis. But they almost exclusively require expensive cofactors such as NADP+, the oxidized form of the biological cofactor nicotinamide adenine dinucleotide phosphate. The reagent currently costs about $22,000 per mole. Enzymes that can recycle it from its reduced form, NADPH, have drawbacks that have limited their use, such as low activity, unwanted by-product generation, and short lifetimes. Guided by nature, which uses an NADP+-producing glutathione reductase system to maintain a reducing environment within cells, researchers have now developed a similar system for regenerating NADP+ from NADPH. Rudolf K. Allemann of Cardiff University and coworkers devised the system, which uses an organic-disulfide oxidizing agent and bacterial glutaredoxin and glutathione reductase to regenerate NADP+ (ACS Catal. 2016, DOI: 10.1021/acscatal.6b03061). The primary recurrent cost is for the inexpensive organic disulfides, so the process could reduce the price of NADP+ to about $0.05 per mole, a more than five-order-of-magnitude improvement. The system, which “is superior to all existing methods” for regenerating NADP+, “offers many advantages for commercial and academic users,” the researchers say.
- Chemical & Engineering News
- ISSN 0009-2347
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