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Separations

Chemistry in Pictures: Why so blue?

by Manny Morone
October 8, 2019

20191009lnp20-whyso.jpg
Credit: Jon Grimm

Despite their attractive appearance, these crystals were not Jon Grimm’s desired product. The sample in this test tube was one of the fractions that eluted through a silica gel column that Grimm, a senior scientist in the lab of Luke Lavis at the Howard Hughes Medical Institute’s Janelia Research Campus, used to separate and purify his reaction mixture. Based on the reaction he was running—a Buchwald-Hartwig cross-coupling en route to a fluorescent rhodamine dye, which are used for imaging experiments at Janelia—he thinks the floating crystals are related to the phosphine ligand he added as part of his catalyst, but he did not fully characterize the substance. And although the crystals look like they glow blue, the fluorescence comes mainly from another impurity still in solution; both of which, Grimm assures, drained out of the column far ahead of his expected product.

Submitted by Jon Grimm. This photo is part of our Chromatography Contest sponsored by Restek. Enter the photo contest here, or submit your chromatography photo via Instagram and Twitter using the hashtag #SeparationScene. Follow Grimm on Twitter (@jonathangrimm) and Instagram (@dyerfulchymist).

Do science. Take pictures. Win money. You can also enter our regular monthly photo contest here.

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Chemistry in Pictures: Dyes divided

Meet the Janelia chemist crafting glowing molecular tools for neuroscientists

Chemistry in Pictures: Just add borane.

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