Volume 94 Issue 42 | p. 7 | News of The Week
Issue Date: October 24, 2016 | Web Date: October 19, 2016

Unusual hydrogen bond plays a bigger role in proteins than chemists realized

Interaction affects 94% of all proteins, study shows
Department: Science & Technology
News Channels: Biological SCENE
Keywords: chemical bonding, protein folding, beta sheets
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C5 hydrogen bonds form between the amide hydrogen and the carbonyl oxygen in the same amino acid.
Credit: Nat. Chem. Biol.
Structures showing C5 hydrogen bond in an amino acid.
 
C5 hydrogen bonds form between the amide hydrogen and the carbonyl oxygen in the same amino acid.
Credit: Nat. Chem. Biol.

Since the 1960s, chemists have known about an unusual type of hydrogen bond: one that can form between the amide hydrogen and the carbonyl oxygen in the same amino acid. But it’s been hard to show that this interaction, called the C5 hydrogen bond, matters beyond amino acids, in proteins.

Now, biochemistry professor Ronald T. Raines and graduate student Robert W. Newberry of the University of Wisconsin, Madison, report experimental and computational evidence that this hydrogen bond indeed helps stabilize proteins—significantly in some cases (Nat. Chem. Biol. 2016, DOI: 10.1038/nchembio.2206).

“It has been problematic to quantify the energetic consequence of this H-bond, particularly in systems larger than a dipeptide where numerous other factors come into play,” says Steve Scheiner, a chemistry professor at Utah State University who studies hydrogen bonding. The new work “takes a major step toward unambiguously verifying the presence of this H-bond, as well as offering some quantitative measures of its influence.”

Raines and Newberry used spectroscopic measurements and quantum mechanical calculations of model peptides called tryptophan zippers, which are β hairpins that are 12 amino acids long, to show that the C5 interaction behaves like a hydrogen bond. They then used bioinformatics to analyze a database of protein structures, revealing that the interaction is widespread, affecting ~5% of amino acids and ~94% of all proteins.

The C5 H-bond is strongest in flat structures, which limits where it can occur. “It has to be in β sheets,” Raines says. “Otherwise, the oxygen and hydrogen are too far away from each other.” Raines speculates that this interaction could be important for stabilizing amyloid-forming proteins rich in β sheets, such as those involved in Alzheimer’s disease.

Incorporating this stabilization energy into models of protein folding could help improve predictions of protein structures, Raines says. “We’re very good at predicting the structures of things that are similar to what’s in the database,” he says. Predicting structures from scratch is much harder. “To truly solve the protein folding problem, we need to be much better in our accounting. We need to understand the fundamental forces that lead to a stable structure.”

 
Chemical & Engineering News
ISSN 0009-2347
Copyright © American Chemical Society
Comments
Pinak Chakrabarti (Thu Oct 20 14:20:28 EDT 2016)
We also observed the C5 hydrogen bonded structure, as part of what we call fused-rings motif, which is very wide-spread in proteins, occurring about 4.1 times per 100 residues. Two such motifs when hydrogen bonded to each other can give rise to a new secondary structure, which we call topi. Please see http://www.nature.com/articles/srep31483
Brahma Panda (Sun Oct 30 22:10:03 EDT 2016)
Though weak theses hydrogen bonds are important in structural as well as functional biology.

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